Limited data of Cyclooxygenases (COXs) role in Multiple Myeloma (MM) onset and/or progression are available. Then, seven human MM cell lines (hMMCLs), representative of different disease stages, were tested to evaluate the COXs expression level. Cell viability upon treatments with COX inhibitors alone or in combination with clinically used anti-MM drugs was also determined. All tested drugs exerted a fair antiproliferative activity on hMMCLs and did not induce changes of the two isoenzymes expression extent. Biosynthesis of COXs-mediated PGE2 and TXB2 and NF-κB activation were measured after cell treatment with COX inhibitors and in combination with the anti-MM drugs. No marked synergistic cytotoxic effect was observed by their coadministration. Measuring the PGE2 and TXB2 formed amount, it seems that the used drugs exert their action by a COXs independent mechanism, at least in the chosen hMMCLs and then also in bone marrow stromal cells (BMSCs), derived from MM patient bone marrow aspirates, as an attempt to build the in vivo disease condition. Our data confirmed the permissive microenvironment (BMSCs) role in MM cell proliferation.

Patient Bone Marrow Aspiration to Explore the Cyclooxygenases (COXs) Involvement in Multiple Myeloma

Scilimati A;Miciaccia M;Pati ML;Ferorelli S;Giampietro R;Contino M;Vacca A
Membro del Collaboration Group
;
2021-01-01

Abstract

Limited data of Cyclooxygenases (COXs) role in Multiple Myeloma (MM) onset and/or progression are available. Then, seven human MM cell lines (hMMCLs), representative of different disease stages, were tested to evaluate the COXs expression level. Cell viability upon treatments with COX inhibitors alone or in combination with clinically used anti-MM drugs was also determined. All tested drugs exerted a fair antiproliferative activity on hMMCLs and did not induce changes of the two isoenzymes expression extent. Biosynthesis of COXs-mediated PGE2 and TXB2 and NF-κB activation were measured after cell treatment with COX inhibitors and in combination with the anti-MM drugs. No marked synergistic cytotoxic effect was observed by their coadministration. Measuring the PGE2 and TXB2 formed amount, it seems that the used drugs exert their action by a COXs independent mechanism, at least in the chosen hMMCLs and then also in bone marrow stromal cells (BMSCs), derived from MM patient bone marrow aspirates, as an attempt to build the in vivo disease condition. Our data confirmed the permissive microenvironment (BMSCs) role in MM cell proliferation.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/413269
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