The diversity within Pleurotus has been creating a range of new potential applications with a profound biological and economical value. Pleurotus spp. are, in fact, becoming increasingly attractive for the development of functional food, nutraceuticals and new drugs, due to their potential antioxidant, antimicrobial, antiproliferative, immunomodulatory, anti-inflammatory and antihypertensive properties. About 200 species are currently accepted in this genus, but only a few of them such as P. florida, P. sajor-caju and P. ostreatus are available in the market. The genetic and metabolic diversity within Pleurotus is of main concern for many researchers. Most of the researches were conducted from a taxonomic point of view in order to clarify and disentangle and biochemical markers have been significantly contributing to these issues. In this work, Pleurotus columbinus, Pleurotus ostreatus and Pleurotus eryngii species complex cultivated as mycelium in vitro were studied to determine the effect of different media on the production of secondary metabolites, antimicrobial and antioxidant activity. The metabolomic profile of Pleurotus spp. was evaluated through ultra-performance liquid chromatography mass spectrometry (UHPLC)-QTOF method. The results highlighted that the differential metabolites among M1-M14 Pleurotus samples covered a total of 58 pathways. Comparisons were made between the metabolic profiles of Pleurotus spp. mycelia grown in solid substrates containing PDA and straw, with respect to substrate containing only PDA taken as reference. As major outcome, this study demonstrates that the metabolic pathways are strongly influenced by the chemical composition of the growth substrate. All aqueous extracts from Pleurotus mycelia displayed antimicrobial activity in the concentration range of 1.56 to 200 μg/mL. The antibacterial effects were particularly evident against Escherichia coli (ATCC 10536) (MIC<2.47mg/mL), whereas the dermatophytes Arthroderma curreyi (CCF 5207) and Trichophyton rubrum (CCF 4933) resulted to be the most sensitive with MIC range between 31.49 and 158.74 μg/mL to the mushroom extracts. Further investigations are needed to evaluate whether and how the growth substrate influences the antimicrobial and antioxidant properties of Pleurotus spp.
DIVERSITY OF PLEUROTUS SPP. AND THEIR METABOLITES OF NUTRACEUTICAL AND THERAPEUTIC IMPORTANCE
Colasuonno, PMembro del Collaboration Group
;Gargano, MLMembro del Collaboration Group
;Angelini, PMembro del Collaboration Group
2022-01-01
Abstract
The diversity within Pleurotus has been creating a range of new potential applications with a profound biological and economical value. Pleurotus spp. are, in fact, becoming increasingly attractive for the development of functional food, nutraceuticals and new drugs, due to their potential antioxidant, antimicrobial, antiproliferative, immunomodulatory, anti-inflammatory and antihypertensive properties. About 200 species are currently accepted in this genus, but only a few of them such as P. florida, P. sajor-caju and P. ostreatus are available in the market. The genetic and metabolic diversity within Pleurotus is of main concern for many researchers. Most of the researches were conducted from a taxonomic point of view in order to clarify and disentangle and biochemical markers have been significantly contributing to these issues. In this work, Pleurotus columbinus, Pleurotus ostreatus and Pleurotus eryngii species complex cultivated as mycelium in vitro were studied to determine the effect of different media on the production of secondary metabolites, antimicrobial and antioxidant activity. The metabolomic profile of Pleurotus spp. was evaluated through ultra-performance liquid chromatography mass spectrometry (UHPLC)-QTOF method. The results highlighted that the differential metabolites among M1-M14 Pleurotus samples covered a total of 58 pathways. Comparisons were made between the metabolic profiles of Pleurotus spp. mycelia grown in solid substrates containing PDA and straw, with respect to substrate containing only PDA taken as reference. As major outcome, this study demonstrates that the metabolic pathways are strongly influenced by the chemical composition of the growth substrate. All aqueous extracts from Pleurotus mycelia displayed antimicrobial activity in the concentration range of 1.56 to 200 μg/mL. The antibacterial effects were particularly evident against Escherichia coli (ATCC 10536) (MIC<2.47mg/mL), whereas the dermatophytes Arthroderma curreyi (CCF 5207) and Trichophyton rubrum (CCF 4933) resulted to be the most sensitive with MIC range between 31.49 and 158.74 μg/mL to the mushroom extracts. Further investigations are needed to evaluate whether and how the growth substrate influences the antimicrobial and antioxidant properties of Pleurotus spp.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
