Ochratoxins are fungal secondary metabolites that may contaminate various foods and beverages. The intake of ochratoxins by humans may result in typical syndromes (nefrotoxicity, carcinogenity, teratogenicity and immunotoxicity) and has been associated with Balkan Endemic Nephropathy. In this study the authors describe a simple and highly specific method for the determination of Ochratoxin A in infant milk products and human breast milk. The method involves: the extraction of Ochratoxin A, clean-up with immunoaffinity columns having specific antibodies and quantification using high performance liquid chromatography with fluorescence detection. The minimum detectable concentration values of OTA obtained were appropriate for the analytical findings, designed to detect OTA in low concentrations.
Valutazione di un metodo HPLC_FL per la determinazione di ocratossina A nel latte materno e per l'infanzia
BONERBA, ELISABETTA;BOZZO, GIANCARLO;DI PINTO, ANGELA;CELANO, Gaetano Vitale;TANTILLO, Giuseppina;CECI, Edmondo
2013-01-01
Abstract
Ochratoxins are fungal secondary metabolites that may contaminate various foods and beverages. The intake of ochratoxins by humans may result in typical syndromes (nefrotoxicity, carcinogenity, teratogenicity and immunotoxicity) and has been associated with Balkan Endemic Nephropathy. In this study the authors describe a simple and highly specific method for the determination of Ochratoxin A in infant milk products and human breast milk. The method involves: the extraction of Ochratoxin A, clean-up with immunoaffinity columns having specific antibodies and quantification using high performance liquid chromatography with fluorescence detection. The minimum detectable concentration values of OTA obtained were appropriate for the analytical findings, designed to detect OTA in low concentrations.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.