Rabbit Hemorrhagic Disease Virus (RHDV) and European Brown Hare Syndrome Virus (EBHSV) are two virus belonging to the Lagovirus genus. They cause a hemorrhagic disease in rabbits and hare, respectively. The high mortality (up to 100%), and the great contagious potential of the viruses make them very dangerous for both wild and domestic animals. The viruses have a number of variants, and recently a new RHDV variant has been widely circulating among rabbits, mainly in France. The variability of viruses may cause some problem in the molecular diagnosis. We set up a RT-PCR based method which could widen the efficacy of the existing molecular techniques, as it could detect simultaneously the variants of RHDV, including the French variants, and the EBHSV, furthermore discriminating the two viral species.

RT-PCR based strategy to identify Rabbit Haemorragic Disease Virus (Italy)

Pugliese N;CAMARDA, Antonio
2012-01-01

Abstract

Rabbit Hemorrhagic Disease Virus (RHDV) and European Brown Hare Syndrome Virus (EBHSV) are two virus belonging to the Lagovirus genus. They cause a hemorrhagic disease in rabbits and hare, respectively. The high mortality (up to 100%), and the great contagious potential of the viruses make them very dangerous for both wild and domestic animals. The viruses have a number of variants, and recently a new RHDV variant has been widely circulating among rabbits, mainly in France. The variability of viruses may cause some problem in the molecular diagnosis. We set up a RT-PCR based method which could widen the efficacy of the existing molecular techniques, as it could detect simultaneously the variants of RHDV, including the French variants, and the EBHSV, furthermore discriminating the two viral species.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/69942
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