The quick, easy, cheap, effective, rugged, and safe (QuEChERS) method can be employed for multi-residue analyses instead of traditional extraction methods due to its advantages in terms of extraction time and required equipment. A modified version of the QuEChERS method has been developed for quantifying eight pharmaceuticals belonging to different classes in three real soils with different chemical properties. Firstly, the soils have been polluted with all contaminants and the recoveries were determined by liquid chromatography tandem–mass spectrometry. Due to similar recoveries from the three soils, the validation of the method has been carried out only on a soil by determining linearity, recovery, precision, limit of detection (LOD) and limit of quantification (LOQ) values. A matrix-matched calibration for the soil has been adopted to avoid the matrix effect and three levels of fortification (50, 100 and 500 μg L-1) were used. The recovery of all pharmaceuticals, except for tetracycline, from any soil was between 72 and 113%. In the validation procedure, recoveries of fortified samples ranged from 80 to 99%, the relative standard deviations ranged between 1.2 and 11.8%, and the LOQ between 20 and 36.9 μg kg-1. The results of the present study confirmed the validity of the modified QuEChERS method for the extraction of pharmaceuticals from soils in the range 50-500 μg kg-1.
Validation of a modified QuEChERS method for the extraction of different classes of pharmaceuticals from soils
De Mastro F.
;Cocozza C.;Traversa A.;Cacace C.;Brunetti G.
2022-01-01
Abstract
The quick, easy, cheap, effective, rugged, and safe (QuEChERS) method can be employed for multi-residue analyses instead of traditional extraction methods due to its advantages in terms of extraction time and required equipment. A modified version of the QuEChERS method has been developed for quantifying eight pharmaceuticals belonging to different classes in three real soils with different chemical properties. Firstly, the soils have been polluted with all contaminants and the recoveries were determined by liquid chromatography tandem–mass spectrometry. Due to similar recoveries from the three soils, the validation of the method has been carried out only on a soil by determining linearity, recovery, precision, limit of detection (LOD) and limit of quantification (LOQ) values. A matrix-matched calibration for the soil has been adopted to avoid the matrix effect and three levels of fortification (50, 100 and 500 μg L-1) were used. The recovery of all pharmaceuticals, except for tetracycline, from any soil was between 72 and 113%. In the validation procedure, recoveries of fortified samples ranged from 80 to 99%, the relative standard deviations ranged between 1.2 and 11.8%, and the LOQ between 20 and 36.9 μg kg-1. The results of the present study confirmed the validity of the modified QuEChERS method for the extraction of pharmaceuticals from soils in the range 50-500 μg kg-1.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
