Pyrethrum (Tanacetum cinerarifolium (Trev.) Schultz-Bip.) is a species belonging to the Asteraceae family. It is native to Albania and Dalmatia1 (Heywood, 1976), although currently the main pyrethrum producers on the world market are Kenya and Australia. The importance of this species is due to the pyrethrins, substances of recognised insecticidal value, extracted from the dried flowers. In the last years, there has been an increasing interest in its introduction in cultivation in the Salento area (most southern area of Puglia Region), due to the economic and environmental advantages. Really, the cultivation of pyrethrum in the Salento area had already been suggested at the beginning of the 1900s and some experimental activities were carried out in Puglia in the early 2000s with encouraging results, using seed from Dalmatia2,3. Despite these good results, the supply of propagation material to guarantee the production of plants with good productive and qualitative characteristics is still limited. Therefore, it is considered particularly interesting to undertake research that could contribute to develop a program of seed production and clonal propagation. The aim of the present study was to develop an efficient in vitro propagation protocol for Tanacetum cinerarifolium (Trev.) Schultz-Bip, to obtain a large number of high quality plants. Seeds were germinated in Petri dishes and the seedlings were grown on a nutrient semi-solid growth medium4 supplemented with sucrose (20 mg/l) and three different phytoregulators (Benzylaminopurine (BAP), Thidiazuron (TDZ) and Metatopoline (MT)) to establish the in vitro conditions and to induce the multiplication of the shoots. The growth medium enriched with MT (0.5 mg/l) gave the best mean multiplication index (5). Three different concentrations of indol-3-butyric acid (IBA) were added to the growth medium to induce rooting: 0.1 mg/l, 0.5 mg/l and 1 mg/l. The higher concentration of IBA resulted in a greater number of roots at the expense of their length, while the lower concentration of 0.1 mg/l gave longer but fewer roots. This study developed an effective and reproducible protocol for micropropagation of pyrethrum.
In vitro propagation of Tanacetum cinerariaefolium (Trev.) Schultz-Bip.
De Mastro G.;Ruta C
2022-01-01
Abstract
Pyrethrum (Tanacetum cinerarifolium (Trev.) Schultz-Bip.) is a species belonging to the Asteraceae family. It is native to Albania and Dalmatia1 (Heywood, 1976), although currently the main pyrethrum producers on the world market are Kenya and Australia. The importance of this species is due to the pyrethrins, substances of recognised insecticidal value, extracted from the dried flowers. In the last years, there has been an increasing interest in its introduction in cultivation in the Salento area (most southern area of Puglia Region), due to the economic and environmental advantages. Really, the cultivation of pyrethrum in the Salento area had already been suggested at the beginning of the 1900s and some experimental activities were carried out in Puglia in the early 2000s with encouraging results, using seed from Dalmatia2,3. Despite these good results, the supply of propagation material to guarantee the production of plants with good productive and qualitative characteristics is still limited. Therefore, it is considered particularly interesting to undertake research that could contribute to develop a program of seed production and clonal propagation. The aim of the present study was to develop an efficient in vitro propagation protocol for Tanacetum cinerarifolium (Trev.) Schultz-Bip, to obtain a large number of high quality plants. Seeds were germinated in Petri dishes and the seedlings were grown on a nutrient semi-solid growth medium4 supplemented with sucrose (20 mg/l) and three different phytoregulators (Benzylaminopurine (BAP), Thidiazuron (TDZ) and Metatopoline (MT)) to establish the in vitro conditions and to induce the multiplication of the shoots. The growth medium enriched with MT (0.5 mg/l) gave the best mean multiplication index (5). Three different concentrations of indol-3-butyric acid (IBA) were added to the growth medium to induce rooting: 0.1 mg/l, 0.5 mg/l and 1 mg/l. The higher concentration of IBA resulted in a greater number of roots at the expense of their length, while the lower concentration of 0.1 mg/l gave longer but fewer roots. This study developed an effective and reproducible protocol for micropropagation of pyrethrum.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
