Methods and Results: Two hundred S. aureus isolates from food of animal origin were screened for methicillin resistance by a PCR assay specific for the mecA gene, an oxacillin agar screen test and a cefoxitin disk diffusion test. Six out of 200 strains (3%) were found to be methicillin-resistant Staphylococcus aureus (MRSA) by PCR. The oxacillin agar screen test detected only one of the MRSA isolates (sensitivity of 16 center dot 7%) and mischaracterized three additional strains as MRSA (specificity of 98 center dot 45%). None of the MRSA strains was detected by the cefoxitin test (sensitivity of 0%), while 15 methicillin-susceptible S. aureus (MSSA) strains were misclassified as resistant (specificity of 92 center dot 3%). Fifteen MSSA strains displayed a beta-lactamase hyperproducer-like phenotype. The six MRSA (mecA-positive) strains resembled the characteristics of heteroresistant strains.
Comparison of methods for the detection of methicillin resistance in Staphylococcus aureus isolates from food products. Lett Appl Microbiol. 2007 Nov;45(5):535-9
CORRENTE, Marialaura;MARTELLA, Vito;BELLACICCO, ANNA LUCIA;QUAGLIA, NICOLETTA CRISTIANA;DAMBROSIO, ANGELA;BUONAVOGLIA, Domenico;BUONAVOGLIA, Canio
2007-01-01
Abstract
Methods and Results: Two hundred S. aureus isolates from food of animal origin were screened for methicillin resistance by a PCR assay specific for the mecA gene, an oxacillin agar screen test and a cefoxitin disk diffusion test. Six out of 200 strains (3%) were found to be methicillin-resistant Staphylococcus aureus (MRSA) by PCR. The oxacillin agar screen test detected only one of the MRSA isolates (sensitivity of 16 center dot 7%) and mischaracterized three additional strains as MRSA (specificity of 98 center dot 45%). None of the MRSA strains was detected by the cefoxitin test (sensitivity of 0%), while 15 methicillin-susceptible S. aureus (MSSA) strains were misclassified as resistant (specificity of 92 center dot 3%). Fifteen MSSA strains displayed a beta-lactamase hyperproducer-like phenotype. The six MRSA (mecA-positive) strains resembled the characteristics of heteroresistant strains.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
