The goal of this study is the chemical-physical characterization of a drug delivery system for Photodynamic Therapy (PDT) [1]: Chlorophyll a (Chl a) entrapped in a water-soluble matrix made of Ca2+ cross-linked alginic acid [2]. Chl a, a lipophilic natural pigments, is a molecule with characteristic photosensitising properties usable in PDT. In PDT the photosensitizer, selectively accumulated in malignant tissues, produces, upon light irradiation, reactive oxygen species (ROS) which are responsible for cytotoxicity of neoplastic cells and tumor regression since induce cellular damage via apoptosis, necrosis, or both. The Chl a/alginate microspheres produced were characterized by means of different techniques as UV-Vis absorption and emission spectroscopy, FT-IR spectroscopy, Atomic Force Microscopy, Dynamic Light Scattering and Differential Scanning Calorimetry. Moreover, it was estimated the ROS production, mainly singlet oxygen (1O2) by means of a selective luminescent probe, Singlet Oxygen Sensor Green [3] and in vitro tests were carried out on human adenocarcinoma cells (HT29). [1] R.M. Szeimies, S. Karrer, S. Radakovic-Fijan. J. Am. Acad. Dermatol., 2002, 47, 259-262. [2] G.T. Grant, E.R. Morris, D.A. Rees, P.J.C. Smith, D. Thom. FEBS letters, 1973, 32,195-198. [3] A. Gollmer, J. Arnbjerg, H. Frances, F.H. Blaikie, B.Wett Pedersen, T. Breitenbach, K. Daasbjerg, P.R. Ogilby. Photochemistry and Photobiology, 2011, 87, 671–679.

Chlorophyll/Alginate microparticles for Photodynamic Therapy applications

Paola Semeraro;CHIMIENTI, Guglielmina Alessandra;AGOSTIANO, Angela;COSMA, Pinalysa
2014

Abstract

The goal of this study is the chemical-physical characterization of a drug delivery system for Photodynamic Therapy (PDT) [1]: Chlorophyll a (Chl a) entrapped in a water-soluble matrix made of Ca2+ cross-linked alginic acid [2]. Chl a, a lipophilic natural pigments, is a molecule with characteristic photosensitising properties usable in PDT. In PDT the photosensitizer, selectively accumulated in malignant tissues, produces, upon light irradiation, reactive oxygen species (ROS) which are responsible for cytotoxicity of neoplastic cells and tumor regression since induce cellular damage via apoptosis, necrosis, or both. The Chl a/alginate microspheres produced were characterized by means of different techniques as UV-Vis absorption and emission spectroscopy, FT-IR spectroscopy, Atomic Force Microscopy, Dynamic Light Scattering and Differential Scanning Calorimetry. Moreover, it was estimated the ROS production, mainly singlet oxygen (1O2) by means of a selective luminescent probe, Singlet Oxygen Sensor Green [3] and in vitro tests were carried out on human adenocarcinoma cells (HT29). [1] R.M. Szeimies, S. Karrer, S. Radakovic-Fijan. J. Am. Acad. Dermatol., 2002, 47, 259-262. [2] G.T. Grant, E.R. Morris, D.A. Rees, P.J.C. Smith, D. Thom. FEBS letters, 1973, 32,195-198. [3] A. Gollmer, J. Arnbjerg, H. Frances, F.H. Blaikie, B.Wett Pedersen, T. Breitenbach, K. Daasbjerg, P.R. Ogilby. Photochemistry and Photobiology, 2011, 87, 671–679.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11586/37358
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