Unaspis euonymi (Comstock) (Hemiptera: Diaspididae) is a well-known and invasive armoured scale insect that infests and damages mostly Euonymus spp. (Celastraceae) such as japonicus Thunb. and europaeus L., all around the world. Recently, specimens of the diaspidid were scru- nized for Serraa spp. while searching about the symbiosis between insect and bacteria. Sur- prisingly enough Serraa symbioca was regularly idenfied in many individuals of Unaspis euonymi collected in Sofia (Bulgaria) during September 2013. We idenfied the bacterium by Denaturing Gradient Gel Electrophoresis (DGGE) and subsequent 16S rRNA sequence ana- lyses of about 600bp PCR fragments obtained from total DNA extracted from single and poo- led insects. Our sequences showed 99% of identy with Serraa symbioca (Bacteria: Enterobacteriaceae) strain CWBI-2.3 recently described as a free-living symbiont of the Black Bean Aphid, the Aphis fabae (Scopoli) (Hemiptera: Aphididae). We discuss the meaning of this finding and the need to gather further evidence on the presence of the bacterium in associa- on with the armoured scale in different places and on different host plants.
Does Unaspis euonymi (Comstock) (Hemiptera: Diaspididae) host Serratia symbiotica Moran (Bacteria: Enterobacteriaceae)?
SCRASCIA, MARIA;PAZZANI, Carlo;OLIVA, MARTA;ROBERTO, ROBERTA;PORCELLI, Francesco
2017-01-01
Abstract
Unaspis euonymi (Comstock) (Hemiptera: Diaspididae) is a well-known and invasive armoured scale insect that infests and damages mostly Euonymus spp. (Celastraceae) such as japonicus Thunb. and europaeus L., all around the world. Recently, specimens of the diaspidid were scru- nized for Serraa spp. while searching about the symbiosis between insect and bacteria. Sur- prisingly enough Serraa symbioca was regularly idenfied in many individuals of Unaspis euonymi collected in Sofia (Bulgaria) during September 2013. We idenfied the bacterium by Denaturing Gradient Gel Electrophoresis (DGGE) and subsequent 16S rRNA sequence ana- lyses of about 600bp PCR fragments obtained from total DNA extracted from single and poo- led insects. Our sequences showed 99% of identy with Serraa symbioca (Bacteria: Enterobacteriaceae) strain CWBI-2.3 recently described as a free-living symbiont of the Black Bean Aphid, the Aphis fabae (Scopoli) (Hemiptera: Aphididae). We discuss the meaning of this finding and the need to gather further evidence on the presence of the bacterium in associa- on with the armoured scale in different places and on different host plants.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
