IN VIVO AND IN VITRO EFFECTS OF EPIGALLOCATECHIN GALLATE (EGCG) ON INTESTINAL MOTILITY OF SPONTANEOUSLY HYPERTENSIVE RATS (SHR) POTENZA Maria Assunta, NACCI Carmela, MONTAGNANI Monica and DE SALVIA Maria Antonietta. Dept. Biomedical Sciences and Human Oncology, Medical School, University of Bari, Piazza G. Cesare, 70124 Bari, Italy Anti-hypertensive effects of green tea drinking are mediated by its most abundant catechin, EGCG. EGCG reaches the highest concentrations in the intestine, where it reduces lipids absorption, inhibits angiogenesis and decreases cancer cell proliferation. Whether EGCG may interfere with gastrointestinal motility is not known. The present study investigates the effects of EGCG on intestinal motility of SHR and WKY rats. For in vivo studies, 9-wk old male SHR were administered with EGCG (200 mg/kg/day) or vehicle (n = 5/group) by gavage for 3 weeks. The contractile dose-response to carbachol (0.05 – 5 µM) and the inhibitory response to electrical field stimulation (EFS, 1- 10 Hz, 13 V, 1 msec, 10-sec train duration) from colon and duodenum specimens were measured before and after L-NNA (100 µM), and compared between EGCG- and vehicle-treated SHR. Colonic response to carbachol and duodenal response to L-NNA were respectively reduced and increased (p < 0.05) in EGCG-treated SHR (vs. vehicle). For in vitro studies, the contractile response to carbachol (1.5 µM) and the inhibitory response to EFS (5 Hz) were measured in colonic and duodenal specimens before and after EGCG (100 µM), and results in SHR compared to those obtained in WKY. EGCG significantly reduced colonic response to carbachol only in SHR, whereas a decreased duodenal inhibitory response to EFS (5 Hz) after EGCG was observed in both SHR and WKY. These data suggest that EGCG may influence intestinal motility, and that gastrointestinal side effects might be associated with drinking of green tea, particularly in hypertensive patients.
In vivo and in vitro effects of epigallocatechin gallate on intestinal motility of spontaneously hypertensive rats
POTENZA, MARIA ASSUNTA;NACCI, CARMELA;MONTAGNANI, MONICA;DE SALVIA, Maria Antonietta
2014-01-01
Abstract
IN VIVO AND IN VITRO EFFECTS OF EPIGALLOCATECHIN GALLATE (EGCG) ON INTESTINAL MOTILITY OF SPONTANEOUSLY HYPERTENSIVE RATS (SHR) POTENZA Maria Assunta, NACCI Carmela, MONTAGNANI Monica and DE SALVIA Maria Antonietta. Dept. Biomedical Sciences and Human Oncology, Medical School, University of Bari, Piazza G. Cesare, 70124 Bari, Italy Anti-hypertensive effects of green tea drinking are mediated by its most abundant catechin, EGCG. EGCG reaches the highest concentrations in the intestine, where it reduces lipids absorption, inhibits angiogenesis and decreases cancer cell proliferation. Whether EGCG may interfere with gastrointestinal motility is not known. The present study investigates the effects of EGCG on intestinal motility of SHR and WKY rats. For in vivo studies, 9-wk old male SHR were administered with EGCG (200 mg/kg/day) or vehicle (n = 5/group) by gavage for 3 weeks. The contractile dose-response to carbachol (0.05 – 5 µM) and the inhibitory response to electrical field stimulation (EFS, 1- 10 Hz, 13 V, 1 msec, 10-sec train duration) from colon and duodenum specimens were measured before and after L-NNA (100 µM), and compared between EGCG- and vehicle-treated SHR. Colonic response to carbachol and duodenal response to L-NNA were respectively reduced and increased (p < 0.05) in EGCG-treated SHR (vs. vehicle). For in vitro studies, the contractile response to carbachol (1.5 µM) and the inhibitory response to EFS (5 Hz) were measured in colonic and duodenal specimens before and after EGCG (100 µM), and results in SHR compared to those obtained in WKY. EGCG significantly reduced colonic response to carbachol only in SHR, whereas a decreased duodenal inhibitory response to EFS (5 Hz) after EGCG was observed in both SHR and WKY. These data suggest that EGCG may influence intestinal motility, and that gastrointestinal side effects might be associated with drinking of green tea, particularly in hypertensive patients.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
