Talc(hydratedmagnesiumsilicate)is a physicalcoadjuvantthat can be employedin the productionofextravirginoliveoil to increaseyield.Theadsorbentpropertiesof talc couldhamperDNArecovery,leadingto falsenegativesin DNAanalysis.Theaim of this workwas to verifythe effectof talc additionon oliveoil DNAby targetingfourselectedmicrosatellites.Oliveprocessingtrialswerecarriedout attwo differentlevelsof talc (1 and 2%)and withouttalc (control).DNAextractionyieldand puritylevelwereascertained,and HighResolutionMelting(HRM)analysisof microsatelliteswas subsequentlyappliedto the extractedDNA.Neitherthe DNAextractionyieldnor the A260/230and A260/280ratiosshowedsignificantdifferencesbetweencontroland talc-treatedsamples.Highervaluesof A260/230andloweryieldswereobservedin oliveoils thanin olivepastes.TheDNAmicrosatellitesanalyzedshowedidenticalHRMprofilesin all the samples,excludingany effectof talc andconfirmingthe genetichomogeneityof the olivelot processed(cv.Coratina).Practicalapplications:Highadded-valuemono-cultivarextravirginoliveoils are nicheproductsveryappreciatedby consumers.DNAanalysisis ableto revealmixingwithcultivarsdifferentfromthosedeclaredin the label.Theuse of talc in oliveoil industrieshas progressivelyincreasedto improveyield.Theresultsobtainedprovethat the DNA-basedmethodsset up untilnowto checkthe authenticityofextravirginoliveoil can be effectivelycarriedout also in oils producedby usingtalc.In addition,theresultsare amongthe first applicationsof HRMto the foodsectorand evidencethe feasibilityof thistechniquealongthe oliveoil chainfor checkingbotholivepastesand oils
High resolution melting analysis of DNA microsatellites in olive pastes and virgin olive oils obtained by talc addition
PASQUALONE, Antonella
;DI RIENZO, VALENTINA;MIAZZI, Monica Marilena;FANELLI, VALENTINA;CAPONIO, Francesco;MONTEMURRO, CINZIA
2015-01-01
Abstract
Talc(hydratedmagnesiumsilicate)is a physicalcoadjuvantthat can be employedin the productionofextravirginoliveoil to increaseyield.Theadsorbentpropertiesof talc couldhamperDNArecovery,leadingto falsenegativesin DNAanalysis.Theaim of this workwas to verifythe effectof talc additionon oliveoil DNAby targetingfourselectedmicrosatellites.Oliveprocessingtrialswerecarriedout attwo differentlevelsof talc (1 and 2%)and withouttalc (control).DNAextractionyieldand puritylevelwereascertained,and HighResolutionMelting(HRM)analysisof microsatelliteswas subsequentlyappliedto the extractedDNA.Neitherthe DNAextractionyieldnor the A260/230and A260/280ratiosshowedsignificantdifferencesbetweencontroland talc-treatedsamples.Highervaluesof A260/230andloweryieldswereobservedin oliveoils thanin olivepastes.TheDNAmicrosatellitesanalyzedshowedidenticalHRMprofilesin all the samples,excludingany effectof talc andconfirmingthe genetichomogeneityof the olivelot processed(cv.Coratina).Practicalapplications:Highadded-valuemono-cultivarextravirginoliveoils are nicheproductsveryappreciatedby consumers.DNAanalysisis ableto revealmixingwithcultivarsdifferentfromthosedeclaredin the label.Theuse of talc in oliveoil industrieshas progressivelyincreasedto improveyield.Theresultsobtainedprovethat the DNA-basedmethodsset up untilnowto checkthe authenticityofextravirginoliveoil can be effectivelycarriedout also in oils producedby usingtalc.In addition,theresultsare amongthe first applicationsof HRMto the foodsectorand evidencethe feasibilityof thistechniquealongthe oliveoil chainfor checkingbotholivepastesand oilsFile | Dimensione | Formato | |
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