In the last years, interest has been increasing in the use of mare’s milk for human nutrition also to find a new exploitation for local equine breeds. In particular mare’s milk is important as possible substitute for cow’s milk or as formulas for allergic children. In fact the mare’s milk composition is quite similar to woman’s milk if compared to cow’s milk Kappa casein is the protein that determines the size and the specific function of milk micelles: its cleavage by chymosin is responsible for milk coagulation. No variants of CSN3 have been discovered in any species belonging to the Equidae family yet. The aim of this study was to asses genetic polymorphism at two linked loci in the exon 1 of the CSN3 gene in Murgese horse breed by PCR-RFLP analysis. The overall frequencies of alleles A and G at CSN3/PstI locus were 0.80 and 0.20 respectively and no GG animals were found in the population. At the CSN3/BseYI locus allelic frequencies were 0.76 and 0.24 for allele C and A respectively and no AA horses were detected. The Hardy-Weinberg equilibrium was verified for both SNPs. Combined genotypic frequencies and possible haplotypes frequencies were also reported.
Analysis of two SNPs located in exon 1 of CSN3 gene in Murgese Horse Breed
SELVAGGI, MARIA;PINTO, Francesco;DARIO, Cataldo
2010-01-01
Abstract
In the last years, interest has been increasing in the use of mare’s milk for human nutrition also to find a new exploitation for local equine breeds. In particular mare’s milk is important as possible substitute for cow’s milk or as formulas for allergic children. In fact the mare’s milk composition is quite similar to woman’s milk if compared to cow’s milk Kappa casein is the protein that determines the size and the specific function of milk micelles: its cleavage by chymosin is responsible for milk coagulation. No variants of CSN3 have been discovered in any species belonging to the Equidae family yet. The aim of this study was to asses genetic polymorphism at two linked loci in the exon 1 of the CSN3 gene in Murgese horse breed by PCR-RFLP analysis. The overall frequencies of alleles A and G at CSN3/PstI locus were 0.80 and 0.20 respectively and no GG animals were found in the population. At the CSN3/BseYI locus allelic frequencies were 0.76 and 0.24 for allele C and A respectively and no AA horses were detected. The Hardy-Weinberg equilibrium was verified for both SNPs. Combined genotypic frequencies and possible haplotypes frequencies were also reported.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
