Pathogens exploit different infection st rategies to infect plants and stomat a represent a direct pathway by which microbes can enter the plant. To counter this, guard cells have evolved the ability to detect conserved microbial molecules and close stomata. In our attempts to understa nd the full nature of the interactions that occur between a potential pathogen and its host, we are focussing on stomatal immunity. The plasma membrane receptor FLS2 confers plant immunity through perceptio n of bacterial flagellin (flg22), which tr iggers stomatal closure. Despite the importance of stomatal immunity, the pathways underlying stomatal behavior and their interaction with immunity control remain largely unknown. To addre ss this, we determined the genetic fr amework of stomatal immunity. I will present our high-throughput imaging pipeline of detecting stomatal apertures, and will discuss some of our recent findings of stomatal immunity control. This includes some recent works on ESCRT-I components that are required for plant immunity at the level of stomata closure. Taken to gether, advanced bioimaging allows us to tackle the role of stomatal closure involved in the in teraction between plants and microbes.
Cyclic AMP deficiency simulates a stress condition in tobacco BY-2 cells
VIGGIANO, Luigi;
2013-01-01
Abstract
Pathogens exploit different infection st rategies to infect plants and stomat a represent a direct pathway by which microbes can enter the plant. To counter this, guard cells have evolved the ability to detect conserved microbial molecules and close stomata. In our attempts to understa nd the full nature of the interactions that occur between a potential pathogen and its host, we are focussing on stomatal immunity. The plasma membrane receptor FLS2 confers plant immunity through perceptio n of bacterial flagellin (flg22), which tr iggers stomatal closure. Despite the importance of stomatal immunity, the pathways underlying stomatal behavior and their interaction with immunity control remain largely unknown. To addre ss this, we determined the genetic fr amework of stomatal immunity. I will present our high-throughput imaging pipeline of detecting stomatal apertures, and will discuss some of our recent findings of stomatal immunity control. This includes some recent works on ESCRT-I components that are required for plant immunity at the level of stomata closure. Taken to gether, advanced bioimaging allows us to tackle the role of stomatal closure involved in the in teraction between plants and microbes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
