ABSTRACT - The aim of the study was to compare the effects of slow freezing and vitrification on blastomere/chromatin integrity and energy/oxidative stress parameters of mouse preimplantation embryos. Mouse (4-cell to blastocyst stage) embryos were analyzed as fresh (controls) or after vitrification or slow freezing. Embryo collapsing was found after both slow freezing (P<0.001) and vitrification (P<0.05). Significantly higher rate of blastomere cytofragmentation was found after slow freezing (P<0.001) but it was not observed after vitrification. Chromatin damage and altered mitochondrial (mt) distribution pattern were observed, both after slow freezing (P<0.05) and vitrification (P<0.05). In embryos at the morula stage, mt activity was reduced by slow freezing (P<0.05) but it did not change by vitrification. In embryos at the blastocyst stage, mt activity was reduced by both slow freezing (P<0.05) and vitrification (P<0.05). Intracellular reactive oxygen species (ROS) level was significantly lower in slow-frozen (P<0.05) but higher in vitrified embryos (P<0.05) compared with controls. Mitochondria/ROS colocalization was significantly reduced after slow freezing (P<0.05) but it was not affected by vitrification. In conclusion, this study demonstrates that vitrification is a suitable method to preserve embryo bioenergy/redox parameters.

Cryopreservation procedure affects morphology, chromatin integrity, energy status and intracellular reactive oxygen species (ROS) levels in 4.cell to blastocyst stage mouse embryos with greater damage after slow freezing than vitrification

MARTINO N. A.;DELL'AQUILA, Maria Elena;
2012

Abstract

ABSTRACT - The aim of the study was to compare the effects of slow freezing and vitrification on blastomere/chromatin integrity and energy/oxidative stress parameters of mouse preimplantation embryos. Mouse (4-cell to blastocyst stage) embryos were analyzed as fresh (controls) or after vitrification or slow freezing. Embryo collapsing was found after both slow freezing (P<0.001) and vitrification (P<0.05). Significantly higher rate of blastomere cytofragmentation was found after slow freezing (P<0.001) but it was not observed after vitrification. Chromatin damage and altered mitochondrial (mt) distribution pattern were observed, both after slow freezing (P<0.05) and vitrification (P<0.05). In embryos at the morula stage, mt activity was reduced by slow freezing (P<0.05) but it did not change by vitrification. In embryos at the blastocyst stage, mt activity was reduced by both slow freezing (P<0.05) and vitrification (P<0.05). Intracellular reactive oxygen species (ROS) level was significantly lower in slow-frozen (P<0.05) but higher in vitrified embryos (P<0.05) compared with controls. Mitochondria/ROS colocalization was significantly reduced after slow freezing (P<0.05) but it was not affected by vitrification. In conclusion, this study demonstrates that vitrification is a suitable method to preserve embryo bioenergy/redox parameters.
978-88-907328-0-5
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11586/57464
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