The presence of N- and O-linked glycans as well as fucosylated and sialilatedglycoproteins was investigated in the donkey prostate by lectinhistochemistry. Prostate tissuefragmentswereroutinelyprocessed forhistologicalinvestigation and stained with a panel of twelve HRP-labeledlectins. The cytoplasm of the secretoryepitheliumdidnot express N-linked and fucosylatedglycans, whereasitcontained O-linkedglycans and negativelychargedglycanssuchassialoglycans. The intraluminalcontentlackedα1,3-sialoglycans compared with the epithelium. Thissuggeststhatchanges in the chemicalcomposition of secretory products occur in the lumen of prostate acini. The reduction of negativelychargedglycoproteinscould be involved in fertility of the donkey.
Characterization of glycans expressed in the prostate of donkey Equus asinus
Maria Albrizio
;Mario Cinone;Antonio Ciro Guaricci;Giovanni Michele Lacalandra;Salvatore Desantis
2025-01-01
Abstract
The presence of N- and O-linked glycans as well as fucosylated and sialilatedglycoproteins was investigated in the donkey prostate by lectinhistochemistry. Prostate tissuefragmentswereroutinelyprocessed forhistologicalinvestigation and stained with a panel of twelve HRP-labeledlectins. The cytoplasm of the secretoryepitheliumdidnot express N-linked and fucosylatedglycans, whereasitcontained O-linkedglycans and negativelychargedglycanssuchassialoglycans. The intraluminalcontentlackedα1,3-sialoglycans compared with the epithelium. Thissuggeststhatchanges in the chemicalcomposition of secretory products occur in the lumen of prostate acini. The reduction of negativelychargedglycoproteinscould be involved in fertility of the donkey.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
