The genetic diversity of the cultivated grapevine (Vitis vinifera) is made by an enormous population of varieties. Although sexual reproduction led to an outstanding level of inter-varietal variability, spontaneous somatic mutations have further contributed to shape the kaleidoscope of grape diversity. For centuries, growers have collected and propagated these different selections and labeled them as “clones”, that represent an untapped genetic resource useful for designing effective conservation strategies and improving traits of economic importance in grapevine. This work aims to analyze population structure and adaptation signatures in the genome of 130 clones belonging to six grape varieties from Campania and Apulia, genotyped using a reduced representation sequencing approach. The intra- and inter-clonal genetic diversity was analyzed using metrics such as nucleotide diversity and genetic dissimilarity as well as population structure and fixation index (Fst). Divergent signatures between Campanian and Apulian accessions and among germplasms were detected, some of which were associated with traits important for grape breeding. Overall, this work emphasizes the need to recover the untapped genetic variability that characterizes minor grape varieties and the requirement to preserve and use grapevine genetic resources more efficiently in breeding programs.

Analysis of genetic diversity and signature of selection in the genome of grapevine clones of Apulian and Campanian varieties

Silvia Procino;Valentina Fanelli;Monica Marilena Miazzi;Cinzia Montemurro;Francesca Taranto;
2023-01-01

Abstract

The genetic diversity of the cultivated grapevine (Vitis vinifera) is made by an enormous population of varieties. Although sexual reproduction led to an outstanding level of inter-varietal variability, spontaneous somatic mutations have further contributed to shape the kaleidoscope of grape diversity. For centuries, growers have collected and propagated these different selections and labeled them as “clones”, that represent an untapped genetic resource useful for designing effective conservation strategies and improving traits of economic importance in grapevine. This work aims to analyze population structure and adaptation signatures in the genome of 130 clones belonging to six grape varieties from Campania and Apulia, genotyped using a reduced representation sequencing approach. The intra- and inter-clonal genetic diversity was analyzed using metrics such as nucleotide diversity and genetic dissimilarity as well as population structure and fixation index (Fst). Divergent signatures between Campanian and Apulian accessions and among germplasms were detected, some of which were associated with traits important for grape breeding. Overall, this work emphasizes the need to recover the untapped genetic variability that characterizes minor grape varieties and the requirement to preserve and use grapevine genetic resources more efficiently in breeding programs.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/496101
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact