Disentangling the human gut microbiota composition is a pre-requisite to unveil its involvement in physiological and pathological host states. The human gut microbiota is composed by Prokaryotes (Archaea and Eubacteria), Viruses and small Eukaryotes such as Fungi and Protista. Over the last two decades, analysis of 16S DNA by metabarcoding allowed the convenient and effective investigation of the gut Bacteria populations, unveiling several insights about their compositional and functional features [1]. By contrast, only in recent years the interest in the Eukaryotic microorganisms of the human gut microbiota has begun to emerge. Aim of this work is therefore to deepen the knowledge about the Eukaryotic community of the gut microbiota, with a particular focus on mycobiota, exploiting the metabarcoding data of healthy control samples in publicly available NCBI BioProjects. For this investigation the (Internal Transcribed Spacer) ITS1 DNA barcode was selected because of its superior reliability in comparison with ITS2 [2]. Then, a compositional profile was obtained. Also, a focus about KEGG [3] pathways potentially associated with investigated microbiota was performed.
The healthy control landscape of the human gut microbiota by using ITS1 DNA metabarcoding data
Defazio, Giuseppe;
2023-01-01
Abstract
Disentangling the human gut microbiota composition is a pre-requisite to unveil its involvement in physiological and pathological host states. The human gut microbiota is composed by Prokaryotes (Archaea and Eubacteria), Viruses and small Eukaryotes such as Fungi and Protista. Over the last two decades, analysis of 16S DNA by metabarcoding allowed the convenient and effective investigation of the gut Bacteria populations, unveiling several insights about their compositional and functional features [1]. By contrast, only in recent years the interest in the Eukaryotic microorganisms of the human gut microbiota has begun to emerge. Aim of this work is therefore to deepen the knowledge about the Eukaryotic community of the gut microbiota, with a particular focus on mycobiota, exploiting the metabarcoding data of healthy control samples in publicly available NCBI BioProjects. For this investigation the (Internal Transcribed Spacer) ITS1 DNA barcode was selected because of its superior reliability in comparison with ITS2 [2]. Then, a compositional profile was obtained. Also, a focus about KEGG [3] pathways potentially associated with investigated microbiota was performed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
