In order to analyze the differentiation and genetics distance between Venezuelan Creole pigs, hairlesssamples were taken from individuals from Capanaparo (n = 29), Cunaviche (n = 32), Guadarrama (n = 31), ElSocorro (n = 26), Hato Masaguaral (n = 22), Guayabal (n = 31), as well as Landrace (n = 21), Large White (n = 14),Alentejano (n = 29) and Ibérico (n = 30) reference groups. In the Laboratory of Molecular Genetics, Center forResearch in Agricultural Biotechnology, Institute of Genetics Faculty of Agronomy, Central University of Venezuela,DNA was extracted from the hair follicles. Were used 13 microsatellite markers: S0155, S0215, S0218, S0225, S0227,SW24, SW240, SW632, SW857, SW911, SW936 y SW951. The fragments were amplified by PCR technique, verticalelectrophoresis was performed on polyacrylamide gels, and the fragments was revealed with silver nitrate. The “F”,“GST” statistics was calculated and the multiple correspondence factorial analyses was performed, the DCand DAdistances were obtained and the dendrogram was constructed using neighbor joining methodology. The “FIT” rangedbetween 0.029 and 0.414;“FIS” between -0.126 and 0.414;“FST” between 0.026 and 0.241;“GST” between 0.043 and0.0293. The smallest distances was observed between El Socorro and Arismendi pigs, and these turn close tocommercial breeds. On the other hand, Guayabal, Capanaparo and Cunaviche pigs were located in separated cluster,being the Cunaviche pigs the closest to the Iberian Peninsula pigs. It is conclude that there is subdivision andmoderate differentiation between Venezuelan Creole pig populations, being some groups close to the commercialbreeds and others to pigs from Iberian Peninsula
Diferenciación y distancia genética entre cerdos criollos venezolanos
Vincenzo Landi;
2020-01-01
Abstract
In order to analyze the differentiation and genetics distance between Venezuelan Creole pigs, hairlesssamples were taken from individuals from Capanaparo (n = 29), Cunaviche (n = 32), Guadarrama (n = 31), ElSocorro (n = 26), Hato Masaguaral (n = 22), Guayabal (n = 31), as well as Landrace (n = 21), Large White (n = 14),Alentejano (n = 29) and Ibérico (n = 30) reference groups. In the Laboratory of Molecular Genetics, Center forResearch in Agricultural Biotechnology, Institute of Genetics Faculty of Agronomy, Central University of Venezuela,DNA was extracted from the hair follicles. Were used 13 microsatellite markers: S0155, S0215, S0218, S0225, S0227,SW24, SW240, SW632, SW857, SW911, SW936 y SW951. The fragments were amplified by PCR technique, verticalelectrophoresis was performed on polyacrylamide gels, and the fragments was revealed with silver nitrate. The “F”,“GST” statistics was calculated and the multiple correspondence factorial analyses was performed, the DCand DAdistances were obtained and the dendrogram was constructed using neighbor joining methodology. The “FIT” rangedbetween 0.029 and 0.414;“FIS” between -0.126 and 0.414;“FST” between 0.026 and 0.241;“GST” between 0.043 and0.0293. The smallest distances was observed between El Socorro and Arismendi pigs, and these turn close tocommercial breeds. On the other hand, Guayabal, Capanaparo and Cunaviche pigs were located in separated cluster,being the Cunaviche pigs the closest to the Iberian Peninsula pigs. It is conclude that there is subdivision andmoderate differentiation between Venezuelan Creole pig populations, being some groups close to the commercialbreeds and others to pigs from Iberian PeninsulaI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.