For recovering the epididymal sperm we proceed in three steps: the distal part of each cauda epididymidis was minced, then epididymis were subjected to the “slide” technique, at the end epididymis were sectioned by a scalpel blade and all spermatozoa manually collected by the blade. Sperm suspension was placed on a Leya slide and concentration, the percentage of motile spermatozoa, VAP, VLS, BCF, STR, LIN, VCL, ALH, were assessed by sperm-CASA.
computer assisted semen analysis of epididymal semen collected throughout the year suggest a seasonality in feral male domestic cat
Leoci R.;Lacalandra GM.;Caira M.;Cicirelli V.;Aiudi G.;
2018-01-01
Abstract
For recovering the epididymal sperm we proceed in three steps: the distal part of each cauda epididymidis was minced, then epididymis were subjected to the “slide” technique, at the end epididymis were sectioned by a scalpel blade and all spermatozoa manually collected by the blade. Sperm suspension was placed on a Leya slide and concentration, the percentage of motile spermatozoa, VAP, VLS, BCF, STR, LIN, VCL, ALH, were assessed by sperm-CASA.File in questo prodotto:
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