The effect of culture-rearing conditions on glycoprotein pattern of Senegalese sole Solea senegalensis developing ovarian follicles was investigated by means of the lectin histochemistry. Ovaries from cultured specimens stopped the oocyte development in the late vitellogenic stage, whereas they reached the hydration stage in wild specimens. Compared to ovaries from wild specimens, in cultured S. senegalensis1 the follicular layer showed the appearance of sialic acid (Neu5Ac) linked to Galβ1,3GalNAc in perinucleolus stage and the lack of Neu5Acα2,6Gal/GalNAc residues2, the zona radiata expressed mannosylated glycans in cortical alveoli stage3, the cortical alveoli, during the late vitellogenic phase, revealed the presence of Neu5AcGalβ1,3GalNAc, Neu5Acα2,6Gal/GalNAc as well as asialogalactosides4, yolkglobules expressed mannosilated oligosaccharides only in the late vitelogenic phase5, the oocyte nucleoplasm did not show sialilated and fucosilated glycans. These findings demonstrate that the glycan pattern of fish ovarian follicles is affected by culture-rearing conditions.
Modification of glycan pattern in cultured Senegal sole, Solea senegalensis (Kaup) ovarian follicles.
ACCOGLI, GIANLUCA;DESANTIS, Salvatore
2012-01-01
Abstract
The effect of culture-rearing conditions on glycoprotein pattern of Senegalese sole Solea senegalensis developing ovarian follicles was investigated by means of the lectin histochemistry. Ovaries from cultured specimens stopped the oocyte development in the late vitellogenic stage, whereas they reached the hydration stage in wild specimens. Compared to ovaries from wild specimens, in cultured S. senegalensis1 the follicular layer showed the appearance of sialic acid (Neu5Ac) linked to Galβ1,3GalNAc in perinucleolus stage and the lack of Neu5Acα2,6Gal/GalNAc residues2, the zona radiata expressed mannosylated glycans in cortical alveoli stage3, the cortical alveoli, during the late vitellogenic phase, revealed the presence of Neu5AcGalβ1,3GalNAc, Neu5Acα2,6Gal/GalNAc as well as asialogalactosides4, yolkglobules expressed mannosilated oligosaccharides only in the late vitelogenic phase5, the oocyte nucleoplasm did not show sialilated and fucosilated glycans. These findings demonstrate that the glycan pattern of fish ovarian follicles is affected by culture-rearing conditions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
