Twenty-nine microsatellite markers were used to evaluate genetic diversity and rela-tionships among three Nigerian goat breeds and to compare with one South African and one European goat breeds as outgroups. A total of 244 goats from the Sahel (47), Maradi (47), and West African Dwarf (67) breeds; and as outgroup: Kalahari (47) and Saanen (36) breeds were included. DNA was extracted from blood preserved on FTA Classic cards according to manufacturer’s protocol. The microsatellite regions were amplified using thermal cycler. Mean number of allele (MNA), expected and observed Heterozygousities (He and Ho, respectively), Hardy-Weinberg Equilibrium (HWE) and genetic distances between populations were calculated. A dendogram was constructed to reveal evolutionary trend in the studied breeds. A genetic structure of the populations was performed using STRUCTURE. Genetic diversity was high with MNA per locus ranging from 6.69 to 8.79 for Kalahari and West African Dwarf, respectively. Ho values ranged from 59 % for West African Dwarf to 64.9 % for Saanen. The highest He estimates were observed in the West African Dwarf (70 %). The lowest He (66.5 %) was observed in Saanen population. The Mean Fis values for the studied populations ranged from 0.055 to 0.148 for Kalahari and West Africa Dwarf, respectively. Genetic distances between populations revealed the least genetic relationship between Saanen and Maradi (0.386) and highest between Maradi and Sahel (0.025). The HWE test revealed eighteen, seventeen, thirteen, twenty-three, and twenty-one loci were in HWE (p>0.05) in Maradi, West African Dwarf, Sahel, Saanen, and Kalahari, respectively. A gra-phic representation of the STRUCTURE analysis revealed that Nigerian goats descended from a common ancestor different from South African and European breeds used as outgroups.

Diversidad genética y estructura de población de cabras autóctonas nigerianas usando marcadores microsatélites DNA

Landi, V.;
2015

Abstract

Twenty-nine microsatellite markers were used to evaluate genetic diversity and rela-tionships among three Nigerian goat breeds and to compare with one South African and one European goat breeds as outgroups. A total of 244 goats from the Sahel (47), Maradi (47), and West African Dwarf (67) breeds; and as outgroup: Kalahari (47) and Saanen (36) breeds were included. DNA was extracted from blood preserved on FTA Classic cards according to manufacturer’s protocol. The microsatellite regions were amplified using thermal cycler. Mean number of allele (MNA), expected and observed Heterozygousities (He and Ho, respectively), Hardy-Weinberg Equilibrium (HWE) and genetic distances between populations were calculated. A dendogram was constructed to reveal evolutionary trend in the studied breeds. A genetic structure of the populations was performed using STRUCTURE. Genetic diversity was high with MNA per locus ranging from 6.69 to 8.79 for Kalahari and West African Dwarf, respectively. Ho values ranged from 59 % for West African Dwarf to 64.9 % for Saanen. The highest He estimates were observed in the West African Dwarf (70 %). The lowest He (66.5 %) was observed in Saanen population. The Mean Fis values for the studied populations ranged from 0.055 to 0.148 for Kalahari and West Africa Dwarf, respectively. Genetic distances between populations revealed the least genetic relationship between Saanen and Maradi (0.386) and highest between Maradi and Sahel (0.025). The HWE test revealed eighteen, seventeen, thirteen, twenty-three, and twenty-one loci were in HWE (p>0.05) in Maradi, West African Dwarf, Sahel, Saanen, and Kalahari, respectively. A gra-phic representation of the STRUCTURE analysis revealed that Nigerian goats descended from a common ancestor different from South African and European breeds used as outgroups.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11586/287986
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