The major difficulty in cartilage defects repair is that articular cartilage tissues are subjected to intensive wear, but endowed with a very low turnover in healthy adults. Moreover, complications such as inflammation, infection or implant loosening often occur after cartilage replacement surgery. Therefore, recent efforts are being focused on designing multifunctional materials able to mimic cartilage mechanical performances and support the surrounding material, without undesirable side effects. In this work, hybrid hydrogels, obtained by ionically crosslinked Gellan Gum (GG), enriched with Manuka honey (MH) and Halloysite nanotubes (HNT) are conceived as artificial cartilage implants, due to their inherent proprieties such as biocompatibility, fluid permeability and lubricating capability. These hydrogels show intriguing mechanical performances, never achieved by other natural hydrogels proposed for cartilage applications . MH is further chosen due to its antimicrobial effectiveness, mainly linked to methylglyoxal (MGO) . XPS, ATR-FTIR and HPLC techniques are exploited to perform a deep chemico-physical characterization of the systems, as well as MGO and cations release. Water uptake, rewet ability and degradation behavior of the hydrogels are also monitored. Mechanical compression and stress-relaxation tests evidence compressive moduli >100kPa for all the prepared samples. Finally, in vitro antibacterial effectiveness, against clinical isolates of S.aureus and S.epidermidis, and cytocompatibility on human mesenchymal stem cells (hMSCs) are extensively investigated. All the obtained results encourage the employment of the developed hydrogels as promising implantable cartilage substitutes.  C.W. Peak, J.J. Wilker, G. Schmidt, A review on tough and sticky hydrogels. Colloid Polym. Sci. 291(9) (2013) 2031-2047.  J.M. Stephens, R.C. Schlothauer, B.D. Morris, D. Yang, L. Fearnley, D.R. Greenwood, K.M. Loomes, Phenolic compounds and methylglyoxal in some New Zealand manuka and kanuka honeys. Food Chem. 120 (2010) 78-86.
|Titolo:||GG-MH HNT hybrid multifunctional materials for cartilage repair: development and analytical characterization|
|Data di pubblicazione:||2018|
|Appare nelle tipologie:||4.1 Contributo in Atti di convegno|