Diarrheic fecal specimens collected from porcine herds were screened for the presence of group C rotaviruses using a reverse transcription-polymerase chain reaction (RT-PCR) assay. A total of 188 samples were tested and 54 were positive. When compiled these data with diagnostic results on group A rotaviruses and enteric caliciviruses we found that all but 5 group C rotavirus positive samples contained at least one additional virus. A subset of samples were subjected to nucleotide sequencing. The selected strains showed an unexpectedly wide range of nucleotide sequence heterogeneity (88.6-100%) to each other and to the reference porcine group C rotavirus strain, Cowden. The nucleotide sequence identity to the genuine bovine and human strains were, respectively, 86.8 and 87.2% or less. In conclusion, our study demonstrates that infection with group C rotavirus is frequent in Italian piggeries. The considerable rate of multiple infections requires further studies to investigate the pathogenic potential of group C rotaviruses in pigs, alone or in mixed infection, and raises challenges in the laboratory diagnosis of porcine enteric infections. © 2007 Elsevier B.V. All rights reserved.

Prevalence of group C rotaviruses in weaning and post-weaning pigs with enteritis

Martella, Vito;Lorusso, Eleonora;Bellacicco, Anna Lucia;Decaro, Nicola;Camero, Michele;Bozzo, Giancarlo;Moschidou, Paschalina;Buonavoglia, Canio
2007-01-01

Abstract

Diarrheic fecal specimens collected from porcine herds were screened for the presence of group C rotaviruses using a reverse transcription-polymerase chain reaction (RT-PCR) assay. A total of 188 samples were tested and 54 were positive. When compiled these data with diagnostic results on group A rotaviruses and enteric caliciviruses we found that all but 5 group C rotavirus positive samples contained at least one additional virus. A subset of samples were subjected to nucleotide sequencing. The selected strains showed an unexpectedly wide range of nucleotide sequence heterogeneity (88.6-100%) to each other and to the reference porcine group C rotavirus strain, Cowden. The nucleotide sequence identity to the genuine bovine and human strains were, respectively, 86.8 and 87.2% or less. In conclusion, our study demonstrates that infection with group C rotavirus is frequent in Italian piggeries. The considerable rate of multiple infections requires further studies to investigate the pathogenic potential of group C rotaviruses in pigs, alone or in mixed infection, and raises challenges in the laboratory diagnosis of porcine enteric infections. © 2007 Elsevier B.V. All rights reserved.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/211467
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