Pentachlorophenol (PCP) was largely applied as wood preservative and can residue into the soil. This highly toxic and persistent xenobiotic was investigated to monitor its impact on the soil environment testing its genotoxic damage by means of the comet assay applied on earthworms, herein used as appropriate bio-indicator. A preliminary time-course experiment in artificial soil was carried out in order to define the most suitable adaptation period required from earthworms in presence ofthe xenobiotic. Then, the genotoxic damage was assayed by means of the comet assay in earthworms exposed to a soil spiked with 15 and 150 ppm of PCP. The comet assay evidenced a dose-dependent DNA damage on earthworm coelomocytes.
Evaluation of the genotoxic effects of pentachlorophenol by Comet assay on earthworm coelomocytes
SPAGNUOLO, Matteo;DE LILLO, Enrico;
2010-01-01
Abstract
Pentachlorophenol (PCP) was largely applied as wood preservative and can residue into the soil. This highly toxic and persistent xenobiotic was investigated to monitor its impact on the soil environment testing its genotoxic damage by means of the comet assay applied on earthworms, herein used as appropriate bio-indicator. A preliminary time-course experiment in artificial soil was carried out in order to define the most suitable adaptation period required from earthworms in presence ofthe xenobiotic. Then, the genotoxic damage was assayed by means of the comet assay in earthworms exposed to a soil spiked with 15 and 150 ppm of PCP. The comet assay evidenced a dose-dependent DNA damage on earthworm coelomocytes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
