New conjugates between inulin (INU) and vitamin E (VITE) able to form amphiphilic systems self-assembling in nanostructures thought for biomedical applications such as the therapy of urinary tract affections were prepared. This work, shows the syntheses, characterization and self-assembling properties of the obtained polymeric conjugates (INVITE). The reaction between VITE and INU has been carried out in bulk, without isolation of intermediate products leading to a convenient one-step reaction. To tailor the physical-chemical characteristics of the INVITE bioconjugate, six different INVITE conjugates were obtained by varying the relative amount of bonded VITE respect to INU repeating units. The obtained products were characterized by 1H-NMR, 13C-NMR, FT-IR and DSC. Furthermore, to verify that VITE does not undergo any oxidation during the reactions, UV-VIS analyses have been performed and the ability of the new conjugates to form nanoparticulate systems in water or 0.9 % saline was performed by dynamic light scattering. Furthermore, in the same media, was evaluated the stability of the INVITE nanosystems after incubation at 25 °C up to 12 days, by measuring at different time points their size variation. The degree of derivatization was found not influenced by the temperature, but it mostly depend from the different molar ratios. Interestingly, the introduction of VITE in the inulin backbone resulted almost quantitative. All conjugates resulted self-assembling in water forming nanosystems sized below 100 nm. The nanosystems are stable and do not aggregate after 12 days incubation. These results strongly encourage to prove these systems for drug delivery applications.
New nanostructured self-assemblong inulin-a-tocopherol bioconjugates for biomedical applications
MANDRACCHIA, DELIA;CUTRIGNELLI, ANNALISA;LAQUINTANA, VALENTINO;DENORA, NUNZIO;LOPEDOTA, Angela Assunta;FRANCO, Massimo;
2012-01-01
Abstract
New conjugates between inulin (INU) and vitamin E (VITE) able to form amphiphilic systems self-assembling in nanostructures thought for biomedical applications such as the therapy of urinary tract affections were prepared. This work, shows the syntheses, characterization and self-assembling properties of the obtained polymeric conjugates (INVITE). The reaction between VITE and INU has been carried out in bulk, without isolation of intermediate products leading to a convenient one-step reaction. To tailor the physical-chemical characteristics of the INVITE bioconjugate, six different INVITE conjugates were obtained by varying the relative amount of bonded VITE respect to INU repeating units. The obtained products were characterized by 1H-NMR, 13C-NMR, FT-IR and DSC. Furthermore, to verify that VITE does not undergo any oxidation during the reactions, UV-VIS analyses have been performed and the ability of the new conjugates to form nanoparticulate systems in water or 0.9 % saline was performed by dynamic light scattering. Furthermore, in the same media, was evaluated the stability of the INVITE nanosystems after incubation at 25 °C up to 12 days, by measuring at different time points their size variation. The degree of derivatization was found not influenced by the temperature, but it mostly depend from the different molar ratios. Interestingly, the introduction of VITE in the inulin backbone resulted almost quantitative. All conjugates resulted self-assembling in water forming nanosystems sized below 100 nm. The nanosystems are stable and do not aggregate after 12 days incubation. These results strongly encourage to prove these systems for drug delivery applications.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
