Low density lipoprotein self-assembled layers on gold support, proposed as model for oxidation studies, were subjected to oxidation processes using different oxidative agents: 2,20-Azobis(2methylpropionamidine)dihydrochloride, atmospheric oxygen, and metal-induced oxidation. The freshly prepared and the oxidized layers were characterized by X ray photoelectron spectroscopy (XPS), Fourier-Transformed infrared spectroscopy, and Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-ToF) mass spectrometry to discriminate the effects of oxidative reagents. Data obtained from FTIR and MALDI spectra proved the lipoperoxide formation subsequent to reactive oxygen species attack and the opportunity to use the model to discriminate between oxidation toxicity.

Spectrochemical characterization of thin layer of lipoproteins self-assembled films on solid supports under oxidation process

CIOFFI, NICOLA;TORSI, Luisa;SABBATINI, Luigia;
2011

Abstract

Low density lipoprotein self-assembled layers on gold support, proposed as model for oxidation studies, were subjected to oxidation processes using different oxidative agents: 2,20-Azobis(2methylpropionamidine)dihydrochloride, atmospheric oxygen, and metal-induced oxidation. The freshly prepared and the oxidized layers were characterized by X ray photoelectron spectroscopy (XPS), Fourier-Transformed infrared spectroscopy, and Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-ToF) mass spectrometry to discriminate the effects of oxidative reagents. Data obtained from FTIR and MALDI spectra proved the lipoperoxide formation subsequent to reactive oxygen species attack and the opportunity to use the model to discriminate between oxidation toxicity.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11586/132520
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