An epidemiological survey for canine parvovirus (CPV) was conducted by collecting 615 faecal samples from dogs with diarrhoea in different European countries. Molecular methods showed that CPV-2a was predominant in most countries, followed by CPV-2c and CPV-2b, whereas 30 strains were not characterised. By sequence analysis of the full-length VP2 gene, 20 of these viruses were characterised as CPV-2c mutants having the synonymous mutation A4061G in the probe-binding region that prevented correct strain characterisation. A real-time polymerase chain reaction (PCR) assay using a minor groove binder probe was able to recognise both mutant and classical CPV-2c strains. These results indicate that the emergence of CPVs with mutations affecting the oligonucleotide-binding region needs a continuous update of molecular diagnostic tools in order to detect efficiently those emerging strains.
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|Titolo:||Detection of a canine parvovirus type 2c with a non-coding mutation and its implications for molecular characterisation|
|Data di pubblicazione:||2013|
|Citazione:||Detection of a canine parvovirus type 2c with a non-coding mutation and its implications for molecular characterisation / Decaro, N; Desario, C; Amorisco, F; Losurdo, M; Elia, G; Parisi, A; Ventrella, G; Martella, V; Buonavoglia, C. - In: THE VETERINARY JOURNAL. - ISSN 1090-0233. - 196:3(2013), pp. 555-557.|
|Appare nelle tipologie:||1.1 Articolo in rivista|