A microdialysis fibre based, low volume sampler is described which can be used in flow injection analysis (FIA) when an on-line dilution of the sample and/or removal of high molecular weight interferents is required. This device used in combination with a lactate amperometric biosensor based on lactate oxidase electrochemically immobilised in a bilayer membrane of poly(o-phenylendiamine) and overoxidized poly(pyrrole) permits the extension of the linear range of response up to 10 mM lactate. Combining microdialysis sampling with FIA and amperometric detection at an interference-free and fast-response biosensor, lactate determination in complex media such as serum, milk and yoghurt can be easily achieved with a high sample throughput and no sample pre-treatment.

A microdialysis fibre based sampler for flow injection analysis: Determination of L-lactate in biofluids by an electrochemically synthesised bilayer membrane based biosensor

PALMISANO, Francesco;
1996-01-01

Abstract

A microdialysis fibre based, low volume sampler is described which can be used in flow injection analysis (FIA) when an on-line dilution of the sample and/or removal of high molecular weight interferents is required. This device used in combination with a lactate amperometric biosensor based on lactate oxidase electrochemically immobilised in a bilayer membrane of poly(o-phenylendiamine) and overoxidized poly(pyrrole) permits the extension of the linear range of response up to 10 mM lactate. Combining microdialysis sampling with FIA and amperometric detection at an interference-free and fast-response biosensor, lactate determination in complex media such as serum, milk and yoghurt can be easily achieved with a high sample throughput and no sample pre-treatment.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/96898
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