In this study, the variety of sugar residues in the gut glycoconjugates of Triturus carnifex (Amphibia, Caudata) are investigated by carbohydrate conventional histochemistry and lectin histochemistry. The oesophageal surface mucous cells contained acidic glycoconjugates, with residues of GalNAc, Gal b1,3 GalNAc and (GlcNAc b1,4)n oligomers. The gastric surface cells mainly produced neutral glycoproteins with residues of fucose, Gal b1-3 GalNAc, Gal-aGal, and (GlcNAc b1,4)n oligomers in N- and O-linked glycans, as the glandular mucous neck cells, with residues of mannose/glucose, GalNAc, Gal b1,3 GalNAc, (GlcNAc b1,4)n oligomers and fucose linked a1,6 or terminal a1,3 or a1,4 in O-linked glycans. The oxynticopeptic tubulo-vesicular system contained neutral glycoproteins with N- and O-linked glycans with residues of Gal-aGal, Gal b1-3 GalNAc and (GlcNAc b1,4)n oligomers; Fuc linked a1,2 to Gal, a1,3 to GlcNAc in (poly)lactosamine chains and a1,6 to GlcNAc in N-linked glycans. Most of these glycoproteins probably corresponds to the H+K+-ATPase b-subunit. The intestinal goblet cells contained acidic glycoconjugates, with residues of Gal- NAc, mannose/ glucose, (GlcNAc b1,4)n oligomers and fucose linked a1,2 to Gal in O-linked oligosaccharides. The different composition of the mucus in the digestive tracts may be correlated with its different functions. In fact the presence of abundant sulphation of glycoconjugates, mainly in the oesophagus and intestine, probably confers resistance to bacterial enzymatic degradation of the mucus barrier.

Glycoconjugate histochemistry of digestive tract of Triturus carnifex (Amphibia, Caudata)

MASTRODONATO, MARIA;
2007

Abstract

In this study, the variety of sugar residues in the gut glycoconjugates of Triturus carnifex (Amphibia, Caudata) are investigated by carbohydrate conventional histochemistry and lectin histochemistry. The oesophageal surface mucous cells contained acidic glycoconjugates, with residues of GalNAc, Gal b1,3 GalNAc and (GlcNAc b1,4)n oligomers. The gastric surface cells mainly produced neutral glycoproteins with residues of fucose, Gal b1-3 GalNAc, Gal-aGal, and (GlcNAc b1,4)n oligomers in N- and O-linked glycans, as the glandular mucous neck cells, with residues of mannose/glucose, GalNAc, Gal b1,3 GalNAc, (GlcNAc b1,4)n oligomers and fucose linked a1,6 or terminal a1,3 or a1,4 in O-linked glycans. The oxynticopeptic tubulo-vesicular system contained neutral glycoproteins with N- and O-linked glycans with residues of Gal-aGal, Gal b1-3 GalNAc and (GlcNAc b1,4)n oligomers; Fuc linked a1,2 to Gal, a1,3 to GlcNAc in (poly)lactosamine chains and a1,6 to GlcNAc in N-linked glycans. Most of these glycoproteins probably corresponds to the H+K+-ATPase b-subunit. The intestinal goblet cells contained acidic glycoconjugates, with residues of Gal- NAc, mannose/ glucose, (GlcNAc b1,4)n oligomers and fucose linked a1,2 to Gal in O-linked oligosaccharides. The different composition of the mucus in the digestive tracts may be correlated with its different functions. In fact the presence of abundant sulphation of glycoconjugates, mainly in the oesophagus and intestine, probably confers resistance to bacterial enzymatic degradation of the mucus barrier.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11586/9671
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