Phosphorylation of proteins plays a pivotal role in signal transduction processes, and it is a key regulator of many biological cell functions. Various strategies have been proposed for the study of phosphoproteome; most of them require a multi-step analysis and sophisticated equipment. Here we describe the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis of PBMC phosphoproteome using as preliminary enrichment step a simple phosphoprotein isolation by lanthanum chloride. This strategy can be most certainly applied to study the phosphoproteome of CTLs isolated from PBMCs. The phosphoproteome analysis of PBMCs, as well as of CTLs, may help to reveal the signaling pathways essential to their biological role in health and disease.

Two-dimensional gel electrophoresis approach for CTL phosphoproteome analysis.

GESUALDO, Loreto
2014-01-01

Abstract

Phosphorylation of proteins plays a pivotal role in signal transduction processes, and it is a key regulator of many biological cell functions. Various strategies have been proposed for the study of phosphoproteome; most of them require a multi-step analysis and sophisticated equipment. Here we describe the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis of PBMC phosphoproteome using as preliminary enrichment step a simple phosphoprotein isolation by lanthanum chloride. This strategy can be most certainly applied to study the phosphoproteome of CTLs isolated from PBMCs. The phosphoproteome analysis of PBMCs, as well as of CTLs, may help to reveal the signaling pathways essential to their biological role in health and disease.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/93425
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
social impact