A survey of the canine pantropic coronavirus infection was carried out as part of the EU Canine Pantropic Coronavirus Epidemiological Survey in 2009-2011. The aim was to detect the presence of canine pantropic coronavirus (CPCoV) in Hungarian dog populations. Samples have been taken from a total of 130 dogs, and polymerase chain reaction after reverse transcription (RT-PCR) has been performed. 18 samples from organs and 11 samples from faeces were positive for canine enteric coronavirus (CCoV). Further specific examination of CPCoV with real-time PCR was carried out, 12 from 18 organ samples were positive, while none of the 11 faecal samples. Virus isolation was not successful from the samples. However, from a total of 29 coronavirus positive samples 27 were simultaneously positive for canine parvovirus type 2 (CPV-2), which raises the question if the observed symptoms and lesions were only caused by CPCoV. The data indicate that canine pantropic coronavirus is present in the Hungarian dog populations, and its occurrence is quite high, since 12 of the 130 tested dogs (9.23%) were carrying CPCoV.
Detection of canine pantropic coronavirus infection in Hungary
DECARO, Nicola;
2012-01-01
Abstract
A survey of the canine pantropic coronavirus infection was carried out as part of the EU Canine Pantropic Coronavirus Epidemiological Survey in 2009-2011. The aim was to detect the presence of canine pantropic coronavirus (CPCoV) in Hungarian dog populations. Samples have been taken from a total of 130 dogs, and polymerase chain reaction after reverse transcription (RT-PCR) has been performed. 18 samples from organs and 11 samples from faeces were positive for canine enteric coronavirus (CCoV). Further specific examination of CPCoV with real-time PCR was carried out, 12 from 18 organ samples were positive, while none of the 11 faecal samples. Virus isolation was not successful from the samples. However, from a total of 29 coronavirus positive samples 27 were simultaneously positive for canine parvovirus type 2 (CPV-2), which raises the question if the observed symptoms and lesions were only caused by CPCoV. The data indicate that canine pantropic coronavirus is present in the Hungarian dog populations, and its occurrence is quite high, since 12 of the 130 tested dogs (9.23%) were carrying CPCoV.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
