Purified preparations of grapevine fanleaf virus consist of three serologically indistinguishable centrifugal components, T, M and B, with sedimentation coefficients of 50, 86 and 120 S respectively, and containing 0, 30 and 42% RNA. At equilibrium in CsCl gradients, T, M and B components precipitate. Their buoyant densities are 1.31, 1.41 and 1.49 g/ml, respectively. Infectivity is associated mostly with B component. RNA is single stranded and occurs as two species with mol. wt. of 1.4x106 (RNA 2) and 2.4x106 (RNA I). M particles contain only RNA 2 whereas B particles contain both species. RNA 2 is non infective, some infectivity is associated with preparations of RNA I, and infectivity can increase tenfold when RNA I and RNA 2 are mixed. The capsid contains a single protein species with mol. wt. of about 54000. Comparable data for arabis mosaic and raspberry mosaic viruses are similar. The present cryptogram of grapevine fanleaf virus is R/I:2.4/42+1.4/30:S/S:S/Ne.
Properties of grapevine fanleaf virus
GALLITELLI, Donato;SAVINO, Vito Nicola;
1976-01-01
Abstract
Purified preparations of grapevine fanleaf virus consist of three serologically indistinguishable centrifugal components, T, M and B, with sedimentation coefficients of 50, 86 and 120 S respectively, and containing 0, 30 and 42% RNA. At equilibrium in CsCl gradients, T, M and B components precipitate. Their buoyant densities are 1.31, 1.41 and 1.49 g/ml, respectively. Infectivity is associated mostly with B component. RNA is single stranded and occurs as two species with mol. wt. of 1.4x106 (RNA 2) and 2.4x106 (RNA I). M particles contain only RNA 2 whereas B particles contain both species. RNA 2 is non infective, some infectivity is associated with preparations of RNA I, and infectivity can increase tenfold when RNA I and RNA 2 are mixed. The capsid contains a single protein species with mol. wt. of about 54000. Comparable data for arabis mosaic and raspberry mosaic viruses are similar. The present cryptogram of grapevine fanleaf virus is R/I:2.4/42+1.4/30:S/S:S/Ne.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
