Normal brain tissue from28 individuals and 50 glioma samples were analyzed by real-time QuantitativeMethylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples byMSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (P = .000009 Mann Whitney Test) and frequencies (P = .0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93%; CI95%: 84%–100%) as compared with MSP (64%; 95%CI: 46%– 82%). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.
High specificity of quantitative methylation-specific PCR analysis for MGMT promoter hypermethylation detection in gliomas
POETA, Maria Luana;
2009-01-01
Abstract
Normal brain tissue from28 individuals and 50 glioma samples were analyzed by real-time QuantitativeMethylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples byMSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (P = .000009 Mann Whitney Test) and frequencies (P = .0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93%; CI95%: 84%–100%) as compared with MSP (64%; 95%CI: 46%– 82%). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
