The glycoprotein pattern was investigated by lectin histochemistry in the urothelium lining the urinary bladder of the donkey Equus asinus. Tissue sections were stained with a panel of twelve lectins, in combination with saponification and sialidase digestion (K-s). The urinary bladder urothelium has three distinct layers from the basal zone to the lumen consisting of basal, intermediate and superficial cells (umbrella cells). Cytoplasm of basal cells reacted with SNA, PNA, K-s-PNA, GSA I-B4 and Con A showing glycans ending with Neu5Ac2,6Gal/GalNAc, Neu5AcGal1,3GalNAc, Gal and with terminal/internal Man. The cytoplasm of umbrella cells displayed an increase of Neu5AcGal1,3GalNAc and the appearance of Neu5AcGal1,3GalNAc, Neu5ac2,3Gal1,4GlcNAc and Neu5AcGalNAc residues (MAL II, K-s-SBA and K-s-HPA staining). Scattered umbrella cells were characterized by glycans terminating with GalNAc binding DBA, SBA and HPA. The mucosa forms folds with a crypt-like appearance where the urothelium shows a different pattern of glycans. The bladder luminal surface stained with K-s-PNA, K-s-DBA, KOH-s-SBA, and K-s-HPA displaying a coating of sialoglycoproteins belonging to O-linked glycans (typical secretory moieties). These findings show that different glycosylation patterns exist along the donkey bladder urothelium, and different sub-populations of umbrella cells are present secreting the sialoglycans which constitute the protective gel layer lining the bladder.
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|Titolo:||In situ characterization of glycans in the urothelium of donkey bladder: Evidence of secretion of sialomucins|
|Autori interni:||DESANTIS, Salvatore|
|Data di pubblicazione:||2013|
|Appare nelle tipologie:||1.1 Articolo in rivista|