Bone marrow mesenchymal stem cells (BM-MSCs) in combination with bioceramics have been used to repair bone lesions but the effects of these biomaterials on BM-MSCs are little known. This study reports the changes observed in the ultrastructure of sheep BM-MSCs incubated with silicon-stabilized tricalcium phosphate (TSP). MSCs were isolated from bone marrow of iliac crest and cultured according to Crovace et al. (VCOT, 21, 2008), TSP was added to some cultures and cells were incubated for 10 days. Control and TPS-incubated cells were fixed with GTA, post-fixed in OsO4 and embedded in Epon 812. Control and TSP cultures consisted of differently electron-dense polygonal cells containìng euchromatic nuclei with prominent nucleoli, In control coltures, the electron-lucent cells were characterized by dilated rER cisternae whereas the moderately electron-dense cells showed dark bodies as prominent features, Small blebs and filopodia were present on the surface of light and dark cells, respectively. In TSP cultures, thè light cells showed large surface blebs, peripheral cytoplasm poor in organelles which were packed with contractile filaments around the nucleus, The dark elongated cells displayed pseudopodia and filopodia, peripheral vacuoles, dense bodies, round or elongated mitochondria, These findings demonstrate that TSP modifies differently the ultrastructure of celi types constituting the BM-MSCs of sheep.
Ultrastructural changes induced by silicon-stabilized tricalcium phosphate in cultured sheep bone marrow mesenchimal stem cells
DESANTIS, Salvatore;ACCOGLI, GIANLUCA;RESTA, Leonardo;CROVACE, Antonio
2011-01-01
Abstract
Bone marrow mesenchymal stem cells (BM-MSCs) in combination with bioceramics have been used to repair bone lesions but the effects of these biomaterials on BM-MSCs are little known. This study reports the changes observed in the ultrastructure of sheep BM-MSCs incubated with silicon-stabilized tricalcium phosphate (TSP). MSCs were isolated from bone marrow of iliac crest and cultured according to Crovace et al. (VCOT, 21, 2008), TSP was added to some cultures and cells were incubated for 10 days. Control and TPS-incubated cells were fixed with GTA, post-fixed in OsO4 and embedded in Epon 812. Control and TSP cultures consisted of differently electron-dense polygonal cells containìng euchromatic nuclei with prominent nucleoli, In control coltures, the electron-lucent cells were characterized by dilated rER cisternae whereas the moderately electron-dense cells showed dark bodies as prominent features, Small blebs and filopodia were present on the surface of light and dark cells, respectively. In TSP cultures, thè light cells showed large surface blebs, peripheral cytoplasm poor in organelles which were packed with contractile filaments around the nucleus, The dark elongated cells displayed pseudopodia and filopodia, peripheral vacuoles, dense bodies, round or elongated mitochondria, These findings demonstrate that TSP modifies differently the ultrastructure of celi types constituting the BM-MSCs of sheep.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
