causes canine infectious cyclic thrombocytopenia (CICT). The vector role of Rhipicephalus sang-uineus group ticks has been only suggested, but definitive evidence is lacking. Thisstudy aimed to detect and quantify A. platys DNA in infected dogs and in their respec-tive ticks through a quantitative real-time PCR assay. From March to May 2009, bloodand tick samples from dogs residing in a CICT-endemic area were collected and molec-ularly analysed. Differences (p < 0.05) were detected in the bacterial load betweentick nymphs (3.5 × 10−2± 2.5 × 10−2) and adults (female: 1.2 × 10−1± 1.1 × 10−1; male:9.3 × 10−2± 1.2 × 10−2) and between unengorged (1.1 × 10−1± 2.8 × 10−2) and partially orfully engorged ticks (1.9 × 10−1± 2.7 × 10−2). No difference was found between ticks col-lected from positive (1.1 × 10−1± 0.6 × 10−1) and negative (1.2 × 10−1± 0.3 × 10−1) dogs(p > 0.05). The mean bacterial load detected in positive dogs was lower than that in theirrespective ticks (p > 0.05). This study provides circumstantial evidence of the putative roleof Rhipicephalus sp. I as a vector of this pathogen.

Detection of Anaplasma platys in dogs and Rhipicephalus sanguineus group ticks by a quantitative real-time PCR.

LATROFA, MARIA STEFANIA;GIANNELLI, ALESSIO;DANTAS TORRES, FILIPE;OTRANTO, Domenico
2014-01-01

Abstract

causes canine infectious cyclic thrombocytopenia (CICT). The vector role of Rhipicephalus sang-uineus group ticks has been only suggested, but definitive evidence is lacking. Thisstudy aimed to detect and quantify A. platys DNA in infected dogs and in their respec-tive ticks through a quantitative real-time PCR assay. From March to May 2009, bloodand tick samples from dogs residing in a CICT-endemic area were collected and molec-ularly analysed. Differences (p < 0.05) were detected in the bacterial load betweentick nymphs (3.5 × 10−2± 2.5 × 10−2) and adults (female: 1.2 × 10−1± 1.1 × 10−1; male:9.3 × 10−2± 1.2 × 10−2) and between unengorged (1.1 × 10−1± 2.8 × 10−2) and partially orfully engorged ticks (1.9 × 10−1± 2.7 × 10−2). No difference was found between ticks col-lected from positive (1.1 × 10−1± 0.6 × 10−1) and negative (1.2 × 10−1± 0.3 × 10−1) dogs(p > 0.05). The mean bacterial load detected in positive dogs was lower than that in theirrespective ticks (p > 0.05). This study provides circumstantial evidence of the putative roleof Rhipicephalus sp. I as a vector of this pathogen.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/66873
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