Allopurinol [315-30-0], oxipurinol [2465-59-0], and uric acid [69-93-2] were detd. in human serum and urine by liq. chromatog. with electrochem. detection. In particular the use of a polarog. detector operating in the oxidative mode, whose principle of detection is based on the property of allopurinol, oxipurinol, and uric acid to form insol. anodic films on Hg, is described. The performance of such a detector is compared with that of a glassy carbon-wall jet detector. Different procedures for sample pretreatment were evaluated.
High-performance liquid chromatography with polarographic and voltammetric anodic detection: simultaneous determination of allopurinol, oxipurinol and uric acid in body fluids / F. Palmisano; E. Desimoni; P. G.Zambonin. - In: JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL APPLICATIONS. - ISSN 0378-4347. - 306(1984), pp. 205-214.