Particle size of diet regulates the rabbit performance. Since mammalian gut is particularly susceptible to particle size of diet and sialoglycoconjugates are implicated in multiple functional roles of the intestinal lining epithelium, we investigated the effect of particle size on the morphology and the expression of sialoglycoconjugates in Italian White rabbit gut. Gut fragments (duodenum, caecum, colon) from fattening rabbits fed with fine (2mm) (G1) or coarse (8mm) (G2) particle size of one isonitrogenous and isocaloric diet were fixed in 4% (w/v) neutral formalin and then embedded in paraffin wax. Sections were stained with Haematoxylin-Eosin (for morphometry) and with MAL II, SNA, PNA, KOH-sialidasi (K-s)-PNA, DBA, K-s-DBA (for glycohistochemistry). Morphometry revealed that 1) duodenum villi are more irregularly-shaped and significantly heigher (p<0.05) in G1 samples. Viceversa, in G2 specimens the lining epithelium of caecum and colon is significantly taller (p<0.05) than G1 ones. Lectin histochemistry showed a major presence in G1 samples of glycans terminating 1) with sialic acid (NeuNAc)Galβ1,3GalNAc (K-s-PNA) in duodedum luminal surface and 2) with NeuNAcα2,6Gal/GalNAc (SNA) in duodenum and colon goblet cells. These results suggest that particle size of diet could affect the morphometric and physicochemical properties of fattening rabbit gut.
Morphometric and sialoglycoconjugate changes in the gut of rabbits fed with different size feed
VENTRIGLIA G;DI SUMMA A;TUFARELLI, VINCENZO;DESANTIS, Salvatore
2009-01-01
Abstract
Particle size of diet regulates the rabbit performance. Since mammalian gut is particularly susceptible to particle size of diet and sialoglycoconjugates are implicated in multiple functional roles of the intestinal lining epithelium, we investigated the effect of particle size on the morphology and the expression of sialoglycoconjugates in Italian White rabbit gut. Gut fragments (duodenum, caecum, colon) from fattening rabbits fed with fine (2mm) (G1) or coarse (8mm) (G2) particle size of one isonitrogenous and isocaloric diet were fixed in 4% (w/v) neutral formalin and then embedded in paraffin wax. Sections were stained with Haematoxylin-Eosin (for morphometry) and with MAL II, SNA, PNA, KOH-sialidasi (K-s)-PNA, DBA, K-s-DBA (for glycohistochemistry). Morphometry revealed that 1) duodenum villi are more irregularly-shaped and significantly heigher (p<0.05) in G1 samples. Viceversa, in G2 specimens the lining epithelium of caecum and colon is significantly taller (p<0.05) than G1 ones. Lectin histochemistry showed a major presence in G1 samples of glycans terminating 1) with sialic acid (NeuNAc)Galβ1,3GalNAc (K-s-PNA) in duodedum luminal surface and 2) with NeuNAcα2,6Gal/GalNAc (SNA) in duodenum and colon goblet cells. These results suggest that particle size of diet could affect the morphometric and physicochemical properties of fattening rabbit gut.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
