The chapter on protein engineering has introduced and discussed an overview of the available methods for the modification of enzymes. Starting with the classical directed evolution (DE) technique, which has been applied extensively throughout several different biocatalytic pro- cesses, the reader moves toward the semi-rational, rational, and de novo design of enzymes. Meanwhile, DE is clearly the current industry-leading technology; depending on the under- standing of the particular enzymatic system, and on the available structural information, other techniques such as rational design are also becoming fast and efficient solutions for the devel- opment of new catalysts. This success would not be possible without the constant improve- ments of the computational techniques and the newly developed modeling systems for enzyme engineering.
Biocatalysis for Practitioners: Techniques, Reactions and Applications
Antonino Biundo;
2020-01-01
Abstract
The chapter on protein engineering has introduced and discussed an overview of the available methods for the modification of enzymes. Starting with the classical directed evolution (DE) technique, which has been applied extensively throughout several different biocatalytic pro- cesses, the reader moves toward the semi-rational, rational, and de novo design of enzymes. Meanwhile, DE is clearly the current industry-leading technology; depending on the under- standing of the particular enzymatic system, and on the available structural information, other techniques such as rational design are also becoming fast and efficient solutions for the devel- opment of new catalysts. This success would not be possible without the constant improve- ments of the computational techniques and the newly developed modeling systems for enzyme engineering.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
