The evolution of the adenine nucleotide translocase family

Homologous genes are grouped into families whose evolution may be different in the various organisms. For the variety of the processes and the well-known mechanism of gene gain and gene loss, which takes place in genome evolution, we deal in comparative analyses with a "one-to-many" or a "many-to-many" relationship between homologous genes going from invertebrates to vertebrates. In this scenario, it is important to understand how gene function has been preserved and in addition the innovations originated in a given lineage or species. The phylogenetic relations between gene family members and their molecular clock behavior may be very helpful to elucidate their functional fates in various organisms. This in turn can direct laboratory experiments and practical applications. In order to track the evolutionary history of the ANT gene family, we have collected and analyzed 46 sequences from fungi to mammals. Phylogenetic analyses have been performed on nucleotide and amino acidic sequences which have produced basically the same results. We observe the presence of multiple isoforms both in lower and higher eukaryotic species, thus a "many-to-many" correspondence between genes. The molecular phylogeny of ANT genes, reported in the present study, allows to date the time of divergence of ANT isoforms in various lineages. Furthermore, the logo analysis has been carried out to characterize the conservation features of ANT proteins particularly in their three similar domains originated by duplication. © 2004 Elsevier B.V. All rights reserved.