We used the whole-cell configuration of the patch-clamp technique to investigate modulation of the voltage-dependent K+ conductance of rat Leydig cells by intracellular pH, Hepes, and Na+. pH (range 6.8-7.4) has no effect on current. Removal of Na+ (from 10 to 0 mM) increases the amplitude of K+ current and abolishes the plateau effect on peak current. Reduction of Hepes (from 25 to 10 mM) increases the amplitude of K+ current and, simultaneously, shifts the threshold for current activation to the left, but the plateau of current is still present. Removal of Na+ cancels the potentiating effect of Hepes on frequency of occurrence of time-dependent inactivation of K+ current. We conclude that the blocking effect of Hepes and Na+ on K+ current may presumably involve two distinct mechanisms, but we can not exclude some cooperativity in their ability to inactivate the voltage-gated potassium conductance of rat Leydig cells
Inhibitory effect of internal sodium and Hepes on the voltage-dependent potassium conductance of rat Leydig cells
DESAPHY, Jean Francois;
1996-01-01
Abstract
We used the whole-cell configuration of the patch-clamp technique to investigate modulation of the voltage-dependent K+ conductance of rat Leydig cells by intracellular pH, Hepes, and Na+. pH (range 6.8-7.4) has no effect on current. Removal of Na+ (from 10 to 0 mM) increases the amplitude of K+ current and abolishes the plateau effect on peak current. Reduction of Hepes (from 25 to 10 mM) increases the amplitude of K+ current and, simultaneously, shifts the threshold for current activation to the left, but the plateau of current is still present. Removal of Na+ cancels the potentiating effect of Hepes on frequency of occurrence of time-dependent inactivation of K+ current. We conclude that the blocking effect of Hepes and Na+ on K+ current may presumably involve two distinct mechanisms, but we can not exclude some cooperativity in their ability to inactivate the voltage-gated potassium conductance of rat Leydig cellsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
