Despite several data of influenza infection in dogs, the first natural outbreak of canine influenza virus, closely related to H3N8 equine subtype, dates back to 2004 in Florida. Subsequent studies highlighted the role of dogs in adaptation of H5N1 to mammals and the susceptibility of dogs to different subtypes of influenza. A prevalence study was carried out on 562 sera collected from pet and kennel dogs in the South of Italy. A c-ELISA test was employed and c-ELISA-positive, c-ELISA-doubtful and random c-ELISA-negative samples were also tested in subtype-specific HI test using H3N8 and H3N2 strains. c-ELISA detected a positivity of 3.56%. HI performed with the H3N8 revealed 2 positive samples and when performed with the H3N2, HI revealed 47 positive samples. c-ELISA showed to be a sensitive and specific technique. HI is a specific method only when the test antigen is homologous to the circulating virus and, because nonspecific-hemagglutination inhibitors may be present in dog sera, false positives can result. The study underlines that dogs, for their close contact with humans, must be a target for testing. Furthermore because it remains to be determined how long antibodies to influenza virus persist in canine sera, the observed prevalence might be underestimated.
A POPULATION PREVALENCE STUDIY ON INFLUENZA INFECTION IN DOGS IN SOUTHERN ITALY
PRATELLI, Annamaria;COLAO, VALERIANA
2014-01-01
Abstract
Despite several data of influenza infection in dogs, the first natural outbreak of canine influenza virus, closely related to H3N8 equine subtype, dates back to 2004 in Florida. Subsequent studies highlighted the role of dogs in adaptation of H5N1 to mammals and the susceptibility of dogs to different subtypes of influenza. A prevalence study was carried out on 562 sera collected from pet and kennel dogs in the South of Italy. A c-ELISA test was employed and c-ELISA-positive, c-ELISA-doubtful and random c-ELISA-negative samples were also tested in subtype-specific HI test using H3N8 and H3N2 strains. c-ELISA detected a positivity of 3.56%. HI performed with the H3N8 revealed 2 positive samples and when performed with the H3N2, HI revealed 47 positive samples. c-ELISA showed to be a sensitive and specific technique. HI is a specific method only when the test antigen is homologous to the circulating virus and, because nonspecific-hemagglutination inhibitors may be present in dog sera, false positives can result. The study underlines that dogs, for their close contact with humans, must be a target for testing. Furthermore because it remains to be determined how long antibodies to influenza virus persist in canine sera, the observed prevalence might be underestimated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
