A study is presented of the ←H+/e- stoichiometry for H+ pumping by the cytochrome chain in isolated rat liver mitochondria under level-flow and steady-state conditions. It is shown that the ←H+/e- stoichiometry for the cytochrome chain varies under the influence of the flow rate and transmembrane ΔμH+. The rate-dependence is shown to be associated with cytochrome c oxidase, whose ←H+/e- ratio varies from 0 to 1, whilst the ←H+/c- ratio for the span covered by cytochrome c reductase is invariably 2.
The cytochrome chain of mitochondria exhibits variable H+/e- stoichiometry
Papa S.
;Capitanio N.;Capitanio G.;De Nitto E.;Minuto M.
1991-01-01
Abstract
A study is presented of the ←H+/e- stoichiometry for H+ pumping by the cytochrome chain in isolated rat liver mitochondria under level-flow and steady-state conditions. It is shown that the ←H+/e- stoichiometry for the cytochrome chain varies under the influence of the flow rate and transmembrane ΔμH+. The rate-dependence is shown to be associated with cytochrome c oxidase, whose ←H+/e- ratio varies from 0 to 1, whilst the ←H+/c- ratio for the span covered by cytochrome c reductase is invariably 2.File in questo prodotto:
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