Objective: We report on a case of Tuberous Sclerosis (TS) with gingival angiofibromatosis (GA), diagnosed by histopathological analysis with Confocal Laser Scanning Microscopy (CLSM) and treated with High-Power Diode Laser gingivectomy. Case presentation: The patient underwent gingivectomy and gingivoplasty with High-Power Diode Laser in pulsed modality and the surgical sample was formalin-fixed, paraffin-embedded and stained with hematoxylin-eosin and Pricrosirius red. Results: Microscopically, thickened acanthotic epithelium with elongated rete ridges, densely packed, whorly collagen fibers, fibroblasts, variably sized vascular structures, and a few chronic inflammatory cells were detected. At CLSM examination, (Nikon Eclipse E-600 with green/red Laser inducing fluorescence) the collagen fibers, showing intense fluorescence, also manifested variable spatial orientation, due to cross-links among the bundles, ad typical of fibromatosis. Also, variably sized blood vessels and large and polygonal interstitial cells displayed fluorescence of lower intensity. The vascular component consisted of small groups of venous-like structures, frequently showing dilated lumina, thin walls and plump endothelial lining. Conclusions: The histopathological analysis with CLSM of GA occurring in TS highlightes distinctive features, such as low fluorescence areas and a typical vascular component which may represent distinctive features of such lesion.
Tuberous Sclerosis: histological analysis with Confocal Laser Scanning Microscope of Gingival Angiofibromatosis
LACAITA, Maria Grazia;CORSALINI, Massimo;
2013-01-01
Abstract
Objective: We report on a case of Tuberous Sclerosis (TS) with gingival angiofibromatosis (GA), diagnosed by histopathological analysis with Confocal Laser Scanning Microscopy (CLSM) and treated with High-Power Diode Laser gingivectomy. Case presentation: The patient underwent gingivectomy and gingivoplasty with High-Power Diode Laser in pulsed modality and the surgical sample was formalin-fixed, paraffin-embedded and stained with hematoxylin-eosin and Pricrosirius red. Results: Microscopically, thickened acanthotic epithelium with elongated rete ridges, densely packed, whorly collagen fibers, fibroblasts, variably sized vascular structures, and a few chronic inflammatory cells were detected. At CLSM examination, (Nikon Eclipse E-600 with green/red Laser inducing fluorescence) the collagen fibers, showing intense fluorescence, also manifested variable spatial orientation, due to cross-links among the bundles, ad typical of fibromatosis. Also, variably sized blood vessels and large and polygonal interstitial cells displayed fluorescence of lower intensity. The vascular component consisted of small groups of venous-like structures, frequently showing dilated lumina, thin walls and plump endothelial lining. Conclusions: The histopathological analysis with CLSM of GA occurring in TS highlightes distinctive features, such as low fluorescence areas and a typical vascular component which may represent distinctive features of such lesion.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
