The performance of enzyme-coupled spectrophotometric assay of protein kinases and its merits as compared with the radioisotopic method in characterizing functional activity of protein kinases are described. Kinetic parameters of protein kinase C and protein kinase A so obtained are presented. General application of the spectrophotometric assay in the study of protein kinases is recommended.
SPECTROPHOTOMETRIC DETERMINATION OF FUNCTIONAL-CHARACTERISTICS OF PROTEIN-KINASES WITH COUPLED ENZYMATIC ASSAY
SARDANELLI, Anna Maria;
1991-01-01
Abstract
The performance of enzyme-coupled spectrophotometric assay of protein kinases and its merits as compared with the radioisotopic method in characterizing functional activity of protein kinases are described. Kinetic parameters of protein kinase C and protein kinase A so obtained are presented. General application of the spectrophotometric assay in the study of protein kinases is recommended.File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
