Dendritic cells (DCs) are the most potent antigen-presenti ng cells able to trigger the adaptive immune response to specific antigens. When non-self -antigens are captured, DCs switch from an “immature” to a “mature” state to fulfill th eir function. Among the several surface proteins involved in DCs maturation, the ro le of aquaporins (AQPs) is still poorly understood. Here we investigated the expressi on profile of Aqps in murine bone marrow derived dendritic cells (BMDCs). Among the Aqps analyzed, Aqp9 was the most expressed by DCs. Its expression level was significa ntly upregulated 6h following LPS exposure. Chemical inhibition of Aqp9 led to a decreased inflammatory cytokines secretion. BMDCs from AQP9-KO mice release lower amount of inflammatory cytokines and chemokines and increased release of IL-10. De spite the reduced release of inflammatory cytokines, Aqp9-KO mice were not protected f rom DSS induced colitis. All together, our data indicate that AQP9 blockade can be an e fficient strategy to reduce DCs inflammatory response but it is not sufficient to protect f rom acute inflammatory insults such as DSS induced colitis.

Corrigendum: Aquaporin-9 contributes to the maturation process and inflammatory cytokine secretion on murine dendritic cells.

De Santis S;Serino G;Gena P;Verna G;Cataldo I;Giannelli G;Calamita G;
2019-01-01

Abstract

Dendritic cells (DCs) are the most potent antigen-presenti ng cells able to trigger the adaptive immune response to specific antigens. When non-self -antigens are captured, DCs switch from an “immature” to a “mature” state to fulfill th eir function. Among the several surface proteins involved in DCs maturation, the ro le of aquaporins (AQPs) is still poorly understood. Here we investigated the expressi on profile of Aqps in murine bone marrow derived dendritic cells (BMDCs). Among the Aqps analyzed, Aqp9 was the most expressed by DCs. Its expression level was significa ntly upregulated 6h following LPS exposure. Chemical inhibition of Aqp9 led to a decreased inflammatory cytokines secretion. BMDCs from AQP9-KO mice release lower amount of inflammatory cytokines and chemokines and increased release of IL-10. De spite the reduced release of inflammatory cytokines, Aqp9-KO mice were not protected f rom DSS induced colitis. All together, our data indicate that AQP9 blockade can be an e fficient strategy to reduce DCs inflammatory response but it is not sufficient to protect f rom acute inflammatory insults such as DSS induced colitis.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/227452
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 3
  • ???jsp.display-item.citation.isi??? 3
social impact