A new gemycircularvirus sequence was obtained from total DNAs extracted from olive leaves and twigs tissues. Its complete genome consists of a single-stranded circular DNA of 2,145 nt, whose sequence was obtained by rolling circle amplification (RCA). Genome analysis identified three open reading frames, sharing homologies with the coat and replication-associated proteins, these latter in the anti-parallel strand, of known gemycircularvirus species. Search for homologies showed that the circular ssDNA sequence is distantly related to other gemycircularviruses thus originating from a new virus species, for which the name olive-associated gemycircularvirus 1 (OaGV1) is proposed. A survey in two different olive-growing areas of the Apulian region (Southern Italy), showed a limited distribution of OaGV1.

Molecular characterisation of a novel gemycircularvirus associated with olive trees in Italy

Antelmi, Ilaria;Sion, Valentina;Nigro, Franco
Conceptualization
;
2019

Abstract

A new gemycircularvirus sequence was obtained from total DNAs extracted from olive leaves and twigs tissues. Its complete genome consists of a single-stranded circular DNA of 2,145 nt, whose sequence was obtained by rolling circle amplification (RCA). Genome analysis identified three open reading frames, sharing homologies with the coat and replication-associated proteins, these latter in the anti-parallel strand, of known gemycircularvirus species. Search for homologies showed that the circular ssDNA sequence is distantly related to other gemycircularviruses thus originating from a new virus species, for which the name olive-associated gemycircularvirus 1 (OaGV1) is proposed. A survey in two different olive-growing areas of the Apulian region (Southern Italy), showed a limited distribution of OaGV1.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/227189
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