Purpose: Phenolic compounds (PC) of virgin olive oil exert several biochemical and pharmacological beneficial effects. Some dietary PC seem to prevent/improve obesity and metabolic-related disorders such as non-alcoholic fatty liver disease (NAFLD). We investigated the possible effects of PC extracted from olive pomace (PEOP) and of the main single molecules present in the extract (tyrosol, apigenin, oleuropein, p-coumaric and caffeic acid) in protecting hepatocytes and endothelial cells against triglyceride accumulation and oxidative stress. Methods: Rat hepatoma and human endothelial cells were exposed to a mixture of oleate/palmitate to mimic the condition of NAFLD and atherosclerosis, respectively. Then, cells were incubated for 24 h in the absence or in the presence of PC or PEOP. Different parameters were evaluated, such as lipid accumulation and oxidative stress-related markers. Results: In hepatic cells, expression of peroxisome proliferator-activated receptors (PPARs) and of stearoyl-CoA desaturase 1 (SCD-1) were assessed as index of lipid metabolism. In endothelial cells, expression of intercellular adhesion molecule-1 (ICAM-1), activation of nuclear factor kappa-B (NF-kB), release of nitric oxide (NO), and wound-healing rate were assessed as index of inflammation. Conclusion: PEOP extract ameliorated hepatic lipid accumulation and lipid-dependent oxidative imbalance thus showing potential applications as therapeutic agent tuning down hepatosteatosis and atherosclerosis.
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|Titolo:||Polyphenolic extract attenuates fatty acid-induced steatosis and oxidative stress in hepatic and endothelial cells|
PORTINCASA, Piero (Corresponding)
|Data di pubblicazione:||2018|
|Citazione:||Polyphenolic extract attenuates fatty acid-induced steatosis and oxidative stress in hepatic and endothelial cells / Vergani, Laura; Vecchione, Giulia; Baldini, Francesca; Grasselli, Elena; Voci, Adriana; Portincasa, Piero; Ferrari, Pier Francesco; Aliakbarian, Bahar; Casazza, Alessandro A.; Perego, Patrizia. - In: EUROPEAN JOURNAL OF NUTRITION. - ISSN 1436-6207. - 57:5(2018), pp. 1793-1805.|
|Appare nelle tipologie:||1.1 Articolo in rivista|