The phytopathogen Pseudomonas syringae pv. actinidiae (Psa) is the causal agent of bacterial canker of kiwifruit. In the last years, it has caused severe economic losses to Actinidia spp. cultivations, mainly in Italy and New Zealand. Conventional strategies adopted did not provide adequate control of infection. Phage therapy may be a realistic and safe answer to the urgent need for novel antibacterial agents aiming to control this bacterial pathogen. In this study, we described the isolation and characterization of two bacteriophages able to specifically infect Psa. ϕPSA1, a member of the Siphoviridae family, is a temperate phage with a narrow host range, a long latency, and a burst size of 178; ϕPSA2 is a lytic phage of Podoviridae family with a broader host range, a short latency, a burst size of 92 and a higher bactericidal activity as determined by the TOD value. The genomic sequence of ϕPSA1 has a length of 51,090bp and a low sequence homology with the other siphophages, whereas ϕPSA2 has a length of 40472bp with a 98% homology with Pseudomonas putida bacteriophage gh-1. Of the two phages examined, ϕPSA2 may be considered as a candidate for phage therapy of kiwifruit disease, while ϕPSA1 seems specific toward the recent outbreak's isolates and could be useful for Psa typing.

Isolation and partial characterization of bacteriophages infecting Pseudomonas syringae pv. actinidiae, causal agent of kiwifruit bacterial canker

D'ADDABBO, PIETRO;FREZZA, DOMENICO;THALLER, MARIA CRISTINA
2014-01-01

Abstract

The phytopathogen Pseudomonas syringae pv. actinidiae (Psa) is the causal agent of bacterial canker of kiwifruit. In the last years, it has caused severe economic losses to Actinidia spp. cultivations, mainly in Italy and New Zealand. Conventional strategies adopted did not provide adequate control of infection. Phage therapy may be a realistic and safe answer to the urgent need for novel antibacterial agents aiming to control this bacterial pathogen. In this study, we described the isolation and characterization of two bacteriophages able to specifically infect Psa. ϕPSA1, a member of the Siphoviridae family, is a temperate phage with a narrow host range, a long latency, and a burst size of 178; ϕPSA2 is a lytic phage of Podoviridae family with a broader host range, a short latency, a burst size of 92 and a higher bactericidal activity as determined by the TOD value. The genomic sequence of ϕPSA1 has a length of 51,090bp and a low sequence homology with the other siphophages, whereas ϕPSA2 has a length of 40472bp with a 98% homology with Pseudomonas putida bacteriophage gh-1. Of the two phages examined, ϕPSA2 may be considered as a candidate for phage therapy of kiwifruit disease, while ϕPSA1 seems specific toward the recent outbreak's isolates and could be useful for Psa typing.
File in questo prodotto:
File Dimensione Formato  
Journal of Basic Microbiology - 2014 - Di Lallo - Isolation and partial characterization of bacteriophages infecting.pdf

accesso aperto

Descrizione: Articolo principale
Tipologia: Documento in Versione Editoriale
Licenza: Creative commons
Dimensione 757.64 kB
Formato Adobe PDF
757.64 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/196815
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 62
  • ???jsp.display-item.citation.isi??? 60
social impact