We describe an improved real-time PCR assay (designated as“Leishmania-FAST15”) for the detection andquantification ofLeishmania infantumandLeishmania braziliensiskinetoplast DNA minicircles in canineblood samples. The analytical sensitivity of this technique is 0.1 fg of DNA, which is equivalent to 0.002parasite per reaction. This assay uses a small reaction volume (15ml) and is rapid to perform, with theresults being available in less than 34 min. This improved assay might also be suitable for detecting andquantifyingL. infantumandL. braziliensisDNA in other tissues, such as bone marrow and lymph nodes

Leishmania-FAST15: A rapid, sensitive and low-cost real-time PCR assay for the detection of Leishmania infantum and Leishmania braziliensis kinetoplast DNA in canine blood samples

DANTAS TORRES, FILIPE
;
OTRANTO, Domenico;Figueredo, Luciana Aguiar
2017-01-01

Abstract

We describe an improved real-time PCR assay (designated as“Leishmania-FAST15”) for the detection andquantification ofLeishmania infantumandLeishmania braziliensiskinetoplast DNA minicircles in canineblood samples. The analytical sensitivity of this technique is 0.1 fg of DNA, which is equivalent to 0.002parasite per reaction. This assay uses a small reaction volume (15ml) and is rapid to perform, with theresults being available in less than 34 min. This improved assay might also be suitable for detecting andquantifyingL. infantumandL. braziliensisDNA in other tissues, such as bone marrow and lymph nodes
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/185230
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